Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

antibody

Cas9 monoclonal antibody (7A9)

ENZ-ABS294-0100

100 µg

monoclonal

mouse

nonconjugated

7A9

western blot, immunocytochemistry, immunoprecipitation

ENZ-ABS294-0100

Cas9 monoclonal antibody (7A9)

Mouse

Monoclonal

Antibody

100 µg

7A9

IgG1κ

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. Cas9 is used to interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. This is accomplished by unwinding foreign DNA and checking for if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. Cas9 is becoming a prominent tool in the field of genome editing because it can cleave nearly any sequence complementary to the guide RNA. Because the target specificity of Cas9 stems from the guide RNA, engineering Cas9 to target new DNA is straightforward, and versions of Cas9 that bind but do not cleave cognate DNA can be used to control transcriptional activation and repression.

CRISPR-associated endonuclease Cas9/Csn1, CRISPR associated protein 9, SpyCas9

N-terminal fragment of Streptococcus pyogenes Cas9.

Recognizes both Cas9 and dCas9 (nuclease deficient Cas9).

Liquid. In PBS containing 0.02% sodium azide.

IF, IP, WB

-20°C