BIO-PROBE® Cytomegalovirus probe | Enzo Life Sciences ENZ-40835 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

other

product name :

BIO-PROBE® Cytomegalovirus probe

catalog :

ENZ-40835

quantity :

2 µg

citations: 10

Reference
Bellas C, Mampaso F, Fraile G, Molina A, Bricio T, Cuesta C. Detection of Epstein-Barr genome in the lymph nodes of Hodgkin's disease. Postgrad Med J. 1993;69:916-9 pubmed
Brigati D, Myerson D, Leary J, Spalholz B, Travis S, Fong C, et al. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology. 1983;126:32-50 pubmed
Langer P, Waldrop A, Ward D. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci U S A. 1981;78:6633-7 pubmed
Grody W, Cheng L, Lewin K. In situ viral DNA hybridization in diagnostic surgical pathology. Hum Pathol. 1987;18:535-43 pubmed
Hilborne L, Nieberg R, Cheng L, Lewin K. Direct in situ hybridization for rapid detection of cytomegalovirus in bronchoalveolar lavage. Am J Clin Pathol. 1987;87:766-9 pubmed
Unger E, Budgeon L, Myerson D, Brigati D. Viral diagnosis by in situ hybridization. Description of a rapid simplified colorimetric method. Am J Surg Pathol. 1986;10:1-8 pubmed
Henderly D, Atalla L, Freeman W, Rao N. Demonstration of cytomegalovirus retinitis by in situ DNA hybridization. Retina. 1988;8:177-81 pubmed
Hashimoto H, Muller H, Muller F, Schmidts H, Stutte H. In situ hybridization analysis of cytomegalovirus lytic infection in Kaposi's sarcoma associated with AIDS. A study of 14 autopsy cases. Virchows Arch A Pathol Anat Histopathol. 1987;411:441-8 pubmed
Montalban C, Bellas C, Rodriguez Garcia J, Aguado M, Fernández Muñoz R. Non-Hodgkin's lymphoma after prolonged remission of Hodgkin's disease in an HIV-infected patient. Ann Oncol. 1991;2:585-7 pubmed
Mampaso F, Bellas C, Molina A, Quereda C, Bricio T, Buzon L. In situ demonstration of Epstein-Barr virus in intravenous drug abusers with generalized lymphadenopathy. Postgrad Med J. 1992;68:739-41 pubmed

product information

Product ID :

ENZ-40835

Catalog Number :

ENZ-40835

Product Name :

BIO-PROBE® Cytomegalovirus probe

Product Type :

Nucleic acid

Size :

2 µg

list of Pubmed id :

8121864, 6302989, 6273878, 3297990, 3035915, 3006523, 2852836, 2821674, 1665342, 1336194

Product Description :

The Cytomegalovirus (CMV) BIO-PROBE® labeled probe is prepared by nick translation of cloned fragments of the CMV Towne strain. The fragments include a total of 30-31 kb of DNA, approximately 20% of the CMV genome. The probe is specific for cytomegalovirus DNA. It does not hybridize to DNAs of other herpesviruses (i.e., herpes simplex virus or Epstein-Barr virus) but it has been found to hybridize to the DNA of CMV clinical isolates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Buffer :

Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.

Uses :

The Cytomegalovirus (CMV) BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes.

Storage :

-20°C