BIO-PROBE® Adenovirus probe | Enzo Life Sciences ENZ-40834 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

other

product name :

BIO-PROBE® Adenovirus probe

catalog :

ENZ-40834

quantity :

2 µg

citations: 4

Reference
Brigati D, Myerson D, Leary J, Spalholz B, Travis S, Fong C, et al. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology. 1983;126:32-50 pubmed
Langer P, Waldrop A, Ward D. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci U S A. 1981;78:6633-7 pubmed
Hyypia T. Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes. J Clin Microbiol. 1985;21:730-3 pubmed
Grody W, Cheng L, Lewin K. In situ viral DNA hybridization in diagnostic surgical pathology. Hum Pathol. 1987;18:535-43 pubmed

product information

Product ID :

ENZ-40834

Catalog Number :

ENZ-40834

Product Name :

BIO-PROBE® Adenovirus probe

Product Type :

Nucleic acid

Size :

2 µg

list of Pubmed id :

6302989, 6273878, 3998103, 3297990

Product Description :

The Adenovirus BIO-PROBE® labeled probe is prepared by nick translation of cloned fragments of the adenovirus 5 genome. The fragments constitute a total of 20 kb of DNA, approximately 56% of the genome. The probe is specific for adenovirus and, in addition to hybridization to adenovirus 5 sequences, the probe will cross hybridize with adenovirus types 4, 7, 11, 20, 40 and 41 due to their considerable sequence homology. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Buffer :

Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.

Uses :

The Adenovirus BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes. Complete kits and protocols are available for in situ hybridization and detection procedures (Enzo’s SIMPLY SENSITIVE® and ULTRA SENSITIVE® enhanced in situ detection systems).

Storage :

-20°C