This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
protein
product name :
Di-ubiquitin (K48-linked)
catalog :
BML-UW9800-0100
quantity :
100 µg
product information
Product ID :
BML-UW9800
Catalog Number :
BML-UW9800-0100
Product Name :
Di-ubiquitin (K48-linked)
Product Type :
Protein
Size :
100 µg
Background :
Polyubiquitinylated target proteins, are a prerequisite for investigating the mechanistic coordination of chain recognition, substrate unfolding, and peptide bond hydrolysis by the 26S proteasome. Ideally these substrates should be homogeneous by several different criteria: overall purity, polyubiquitin chain length, chain linkage, and site of chain ligation to the target protein. Historically, well-defined substrates have been unattainable because of the low purity and abundance of E3 enzymes, as well as the uncontrolled character of chain elongation as catalysed by available E2 and E3 enzymes. To overcome the chain elongation problem, a method using the well-characterized ubiquitin-conjugating enzyme E2-25K (Prod. No. BML-UW9020) to generate Lys48-linked polyubiquitin chains of defined length has been employed. This method avoids uncontrolled polymerization by utilizing, in each round of synthesis, two chain reactants, one reversibly capped at itsproximal, and the other at its distal, terminus. Di-ubiquitin may be used to aid investigation of ubiquitin binding entities with chains of defined length and character.
ALTnames :
Ub2
Purity :
≥95% (SDS-PAGE)
Buffer :
Liquid. In 50mM TRIS pH 7.5, 1mM EDTA and 5mM DTT.
Uses :
Di-ubiquitin may be used to aid investigation of ubiquitin binding entities with chains of defined length and character.
Storage :
-80°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.