Ubiquitin activating enzyme E1 (human), (recombinant) (His-tag) | Enzo Life Sciences BML-UW9410-0050 product information

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product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

Ubiquitin activating enzyme E1 (human), (recombinant) (His-tag)

catalog :

BML-UW9410-0050

quantity :

50 µg

citations: 6

Reference
Tawo R, Pokrzywa W, Kevei E, Akyuz M, Balaji V, Adrian S, et al. The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover. Cell. 2017;169:470-482.e13 pubmed publisher
Teng Y, Rezvani K, De Biasi M. UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP. Biochem Pharmacol. 2015;97:518-530 pubmed publisher
Liu Y, Lear T, Iannone O, Shiva S, Corey C, Rajbhandari S, et al. The Proapoptotic F-box Protein Fbxl7 Regulates Mitochondrial Function by Mediating the Ubiquitylation and Proteasomal Degradation of Survivin. J Biol Chem. 2015;290:11843-52 pubmed publisher
Liu Y, Lear T, Zhao Y, Zhao J, Zou C, Chen B, et al. F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7. Cell Death Dis. 2015;6:e1630 pubmed publisher
Haas A, Warms J, Hershko A, Rose I. Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation. J Biol Chem. 1982;257:2543-8 pubmed
Ciechanover A, Heller H, Katz Etzion R, Hershko A. Activation of the heat-stable polypeptide of the ATP-dependent proteolytic system. Proc Natl Acad Sci U S A. 1981;78:761-5 pubmed

product information

Product ID :

BML-UW9410

Catalog Number :

BML-UW9410-0050

Product Name :

Ubiquitin activating enzyme E1 (human), (recombinant) (His-tag)

Product Type :

Protein

Size :

50 µg

list of Pubmed id :

26265139, 25778398, 25654763, 6277905, 6262770

Product Description :

Ubiquitin activating enzyme E1 is responsible for the first step in ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating with ATP the C-terminal glycine carboxyl group of ubiquitin and, thereafter, linking this residue to the sulphydryl side chain moiety of a cysteine residue in E1 by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.

Background :

Results demonstrate the formation of ubiquitin thioester linked E2 conjugates of the expected size in all TE +ve control reactions. The absence of such conjugates in TE –ve control reactions demonstrates that their formation is ATP dependent (required for E1 activation) and hence derived from the ubiquitin cascade.

Purity :

≥90% (SDS-PAGE)

Buffer :

In 50mM TRIS-hydrochloric acid, containing 1.0mM dithiothreitol.

Storage :

-80°C