SUMO-1 (human), (recombinant) (His-tag) | Enzo Life Sciences BML-UW9195-0500 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

SUMO-1 (human), (recombinant) (His-tag)

catalog :

BML-UW9195-0500

quantity :

500 µg

citations: 6

Reference
Wu Y, Ling T, Lu S, Kuo H, Ho H, Yeh S, et al. Chemotherapeutic sensitivity of testicular germ cell tumors under hypoxic conditions is negatively regulated by SENP1-controlled sumoylation of OCT4. Cancer Res. 2012;72:4963-73 pubmed publisher
Bohren K, Nadkarni V, Song J, Gabbay K, Owerbach D. A M55V polymorphism in a novel SUMO gene (SUMO-4) differentially activates heat shock transcription factors and is associated with susceptibility to type I diabetes mellitus. J Biol Chem. 2004;279:27233-8 pubmed
Tatham M, Jaffray E, Vaughan O, Desterro J, Botting C, Naismith J, et al. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J Biol Chem. 2001;276:35368-74 pubmed
Muller S, Hoege C, Pyrowolakis G, Jentsch S. SUMO, ubiquitin's mysterious cousin. Nat Rev Mol Cell Biol. 2001;2:202-10 pubmed
Rodriguez M, Dargemont C, Hay R. SUMO-1 conjugation in vivo requires both a consensus modification motif and nuclear targeting. J Biol Chem. 2001;276:12654-9 pubmed
Rodriguez M, Desterro J, Lain S, Midgley C, Lane D, Hay R. SUMO-1 modification activates the transcriptional response of p53. EMBO J. 1999;18:6455-61 pubmed

product information

Product ID :

BML-UW9195

Catalog Number :

BML-UW9195-0500

Product Name :

SUMO-1 (human), (recombinant) (His-tag)

Product Type :

Protein

Size :

500 µg

list of Pubmed id :

23002208, 15123604, 11451954, 11265250, 11124955, 10562557

Background :

SUMO (small ubiquitin-related modifier) is a member of a family of ubiquitin-like proteins involved in the regulation of the cellular functions of a wide variety of proteins. Four members of the SUMO family have been described in vertebrates, SUMO-1 and the close homologues SUMO-2 and SUMO-3, with some 50% homology between SUMO-1 and SUMO-2/3. Although a fourth SUMO (SUMO-4) has been reported, there remains considerable debate as to whether this is a real and expressed gene product. The mechanism for SUMO conjugation is analogous to that of the ubiquitin system, relying upon utilisation of E1, E2 and (potentially) E3 cascade enzymes to covalently link the carboxy-terminal of SUMO proteins to specific lysine residues on target proteins via isopeptide bonds. Unlike ubiquitinylation, which leads, inter alia, to a degradative pathway, SUMO modification of target proteins is involved in nuclear protein targeting, formation of sub-nuclear complexes, regulation of transcriptional activities, and control of protein stability. A short sequence containing the consensus Ψ-K-X-D/E (where lysine is the modified amino acid, Ψ is a large hydrophobic residue and X is any amino acid residue) is thought to be necessary for protein SUMOylation to occur, however, SUMOylation has also been observed in cases where the consensus site is not conserved. Conjugation of mature SUMO proteins to specific lysine residues on target proteins via isopeptide bonds, is involved in a range of processes including nuclear protein targeting, formation of sub-nuclear complexes, regulation of transcriptional activities, and control of protein stability.

ALTnames :

Sentrin-1, Small ubiquitin-related modifier 1

Purity :

≥95% (SDS-PAGE)

Buffer :

Liquid. In HEPES, pH 8.0, containing 50mM NaCl, 1mM DTT.

Uses :

In the presence of HeLa Fraction II (Prod. No. BML-HW8605) or a combination of SUMO E1 and E2 (Prod. No. BML-UW9330 and BML-UW9320 respectively), with ATP, Mg2+, and DTT, mature SUMO-1 may be conjugated to soluble proteins. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM, depending upon conditions. Uses:. 1. SUMO-1 modification of specific proteins in vitro. 2. Demonstrate novel proteins are potential targets for SUMOylation under in vitro conditions. 3. Generate substrates for deSUMOylating enzymes.

Storage :

-80°C