UbcH7 (human), (recombinant) (His-tag) | Enzo Life Sciences BML-UW9080-0100 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

UbcH7 (human), (recombinant) (His-tag)

catalog :

BML-UW9080-0100

quantity :

100 µg

citations: 1

Reference
Liu Y, Lear T, Zhao Y, Zhao J, Zou C, Chen B, et al. F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7. Cell Death Dis. 2015;6:e1630 pubmed publisher

product information

Product ID :

BML-UW9080

Catalog Number :

BML-UW9080-0100

Product Name :

UbcH7 (human), (recombinant) (His-tag)

Product Type :

Protein

Size :

100 µg

list of Pubmed id :

25654763

Background :

Ubiquitinylation of proteins constitutes an important cellular mechanism for targeting short-lived proteins for degradation by the 26S proteasome. Three classes of enzymes are involved in the conjugation of ubiquitin to proteins. E1, the ubiquitin activating enzyme, activates ubiquitin through the ATP-dependent formation of a high-energy thiol ester bond between the carboxyl terminus of ubiquitin and the active-site cysteine within E1. This E1-activated ubiquitin is transferred to a cysteine residue of an E2, or ubiquitin-conjugating enzyme (UbC). E2 enzymes, either by themselves or in conjunction with E3 enzymes (ubiquitin ligases), then transfer ubiquitin to target proteins forming stable isopeptide bonds resulting in multi-ubiquitin chain formation. It is the diverse combinations of E2-E3 complexes which are thought to define substrate specificity. UbcH7 is a class I enzyme which functions in the stress response and the control of transcription factors1. The enzyme is ubiquitously expressed with high levels of expression seen in adult muscle2. It has been demonstrated to participate in the ubiquitinylation of p53, c-Fos and NF-κB. UbcH7 is one of two E2s (UbcH5 being the other) with which HECT domain proteins interact with UbcH7 being able to efficiently substitute for UbcH5 in E6-AP-dependent ubiquitinylation.

Purity :

≥95% (SDS-PAGE)

Buffer :

Liquid. In HEPES, pH 7.5, containing 150 mM NaCl and 1.0mM DTT.

Uses :

Typical enzyme concentration to support conjugation in vitro is 100nM to 1µM depending upon conditions.

Storage :

-80°C