This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
protein
product name :
Tetra-ubiquitin (K48-linked)
catalog :
BML-UW8645-0025
quantity :
25 µg
product information
Product ID :
BML-UW8645
Catalog Number :
BML-UW8645-0025
Product Name :
Tetra-ubiquitin (K48-linked)
Product Type :
Protein
Size :
25 µg
Product Description :
Biochemically useful quantities of polyubiquitinylated target proteins are a pre-requisite for dissecting the mechanistic co-ordination of chain recognition, substrate unfolding, and peptide bond hydrolysis by the 26S proteasome. Di-ubiquitin (Ub2) is made by incubating E2-25k (Prod. No. BML-UW9020) with equal concentrations of ubiquitin capped at the future proximal chain terminus (Asp77-ubiquitin) and ubiquitin capped at the future distal chain terminus (K48C-ubiquitin). The proximal or distal terminus of the resulting dimer is deblocked by treatment with Ubiquitin hydrolase L3 (Prod. No. BML-UW9745) or ethyleneimine, respectively. In the second cycle of synthesis, tetra-ubiquitin (Ub4) is made by incubating the two single-deblocked dimers with E2-25k. Following purification by anion and cation exchange chromatography, the distal Cys48 is blocked by reaction with iodoacetamide, which is then removed by dialysis. Ub4 retains Asp77 at its proximal terminus.
ALTnames :
Ub4
Purity :
≥85% (SDS-PAGE)
Buffer :
Liquid, containing 50mM TRIS pH7.5, 1mM EDTA and 5mM DTT.
Uses :
Useful for studying the mechanism of chain recognition, substrate unfolding, and peptide bond hydrolysis by the 26S proteasome.
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.