This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
protein
product name :
Ubiquitin aldehyde, (recombinant)
catalog :
BML-UW8450-0050
quantity :
50 µg
citations: 4
Reference
Lai Y, Li J, Li X, Zou C. Lipopolysaccharide modulates p300 and Sirt1 to promote PRMT1 stability via an SCFFbxl17-recognized acetyldegron. J Cell Sci. 2017;130:3578-3587 pubmed publisher
Liu Y, Lear T, Iannone O, Shiva S, Corey C, Rajbhandari S, et al. The Proapoptotic F-box Protein Fbxl7 Regulates Mitochondrial Function by Mediating the Ubiquitylation and Proteasomal Degradation of Survivin. J Biol Chem. 2015;290:11843-52 pubmed publisher
Liu Y, Lear T, Zhao Y, Zhao J, Zou C, Chen B, et al. F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7. Cell Death Dis. 2015;6:e1630 pubmed publisher
Shaeffer J, Cohen R. Differential effects of ubiquitin aldehyde on ubiquitin and ATP-dependent protein degradation. Biochemistry. 1996;35:10886-93 pubmed
product information
Product ID :
BML-UW8450
Catalog Number :
BML-UW8450-0050
Product Name :
Ubiquitin aldehyde, (recombinant)
Product Type :
Protein
Size :
50 µg
list of Pubmed id :
25778398, 25654763, 8718881
Product Description :
Synthetically modified recombinant ubiquitin. The modification of the C-terminal glycine carboxyl into an aldehyde results in a potent, highly specific inhibitor of all ubiquitin deconjugating enzymes, including ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs) and deubiquitinylating enzymes (DUBs).
ALTnames :
Ub-H
Purity :
≥95% (HPLC)
Buffer :
Liquid. In aqueous solution containing 0.15M HCl.
Uses :
Ubiquitin Aldehyde (recombinant) is useful in the stabilisation of ubiquitin-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts. Inhibition of deubiquitinylating enzyme activity by Ubiquitin Aldehyde (recombinant) can be used to identify and confirm such activity and to determine the inhibition kinetics for a particular enzyme. Recommended concentration for maximal inhibition is 2-5µM. Co-crystallisation of ubiquitin aldehyde with specific deubiquitinylating enzymes (the inhibitor mimics the natural ubiquitin substrate) has also been used to probe enzyme:substrate interactions.
Storage :
+4°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.