Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

protein

Hexa-ubiquitin (linear)

BML-UW0795-0100

100 µg

BML-UW0795-0100

Hexa-ubiquitin (linear)

Protein

100 µg

Linear polyubiquitin chains are useful tools for investigating, amongst other things, the specificity and reactivity of deubiquitinylating enzymes (DUBs) and the recognition and interaction of linear polyubiquitin modified proteins with by ubiquitin binding domain (UBDs) containing proteins.

The post-translational modification of proteins with polyubiquitin chains occurs in a wide range of signalling pathways and is tightly regulated in order to ensure cellular homeostasis. The function, processing and ultimate fate of polyubiquitinylated proteins is thought to be determined by the nature of the linkage between adjacent ubiquitin molecules in the polyubiquitin chain. In addition to lysine-linked polyubiquitin chains the amino terminus of ubiquitin can be used to form head-to-tail polyubiquitin chains, in which the C-terminal Gly of one ubiquitin is conjugated to the N-terminal Met of an adjacent ubiquitin. Such linear polyubiquitin chains are structurally similar to Lys63-linked polyubiquitin, despite the chemical differences between the two linkage types. Several ubiquitin polygenes are encoded in eukaryotic cells and undergo post-translational processing to generate the cellular source of free ubiquitin monomers. However, linear polyubiquitin chains,in which the C-terminal Gly of one ubiquitin is conjugated to the N-terminal Met of an adjacent ubiquitin, have been shown to be assembled in vitro by an E3 ligase complex, known as the linear ubiquitin chain assembly complex (LUBAC), and ubiquitin binding domains (UBDs) with a preference for linear polyubiquitin have been identified in a number of proteins. A possible role for linear polyubiquitin modification in NF-κB pathway activation, involving linear polyubiquitinylation of NEMO, has also been reported. Such observations suggest that linear polyubiquitin modification of proteins may play an important role in cellular processes in addition to that of lysine-linked polyubiquitin protein conjugation.

Ub6

≥95% (SDS-PAGE)

Liquid. In 50mM TRIS, pH 8.0, containing 50mM sodium chloride.

For use in deubiquitinylating enzyme assays and polyubiquitin binding studies. Suggested uses: Deubiquitinylating enzyme substrates (general/linkage specific). Investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins. Linear polyubiquitin studies.

-20°C