Ubiquitin vinyl sulfone, (HA-tag) | Enzo Life Sciences BML-UW0155-0025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

Ubiquitin vinyl sulfone, (HA-tag)

catalog :

BML-UW0155-0025

quantity :

25 µg

citations: 1

Reference
Griffin P, Sexton A, MacNeill L, Iizuka Y, Lee M, Bazzaro M. Method for measuring the activity of deubiquitinating enzymes in cell lines and tissue samples. J Vis Exp. 2015;:e52784 pubmed publisher

product information

Product ID :

BML-UW0155

Catalog Number :

BML-UW0155-0025

Product Name :

Ubiquitin vinyl sulfone, (HA-tag)

Product Type :

Protein

Size :

25 µg

list of Pubmed id :

25993605

Product Description :

For use in the detection and identification of deubiquitinylating enzymes. HA-Ub-VS is a DUB active site directed probe generated by a chemical ligation method, that acts as a potent and irreversible inhibitor of DUBs through covalent modification of the active site and as a specific probe for enzymes with DUB activity. The HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein, facilitates sensitive identification or purification of HA-Ub-VS modified DUBs through recognition by anti-HA antibodies and/or anti-HA-agarose.

ALTnames :

HA-Ub-VS

Purity :

≥95% (SDS-PAGE)

Buffer :

Lyophilized

Uses :

Suggested uses:. 1. Deubiquitinylating enzyme active site-directed probes for detection of novel DUBs. 2. Determination of deubiquitinylating enzyme activity of putative DUBs. 3. Identification of DUBs from biological samples via immunoprecipitation and proteomic methods. 4. Characterization of deconjugating enzymes for Ub/Ubl specificity.

Storage :

-80°C