This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
protein
product name :
Ubiquitin-AMC
catalog :
BML-SE211-0025
quantity :
25 µg
citations: 2
Reference
Mason D, Ek J, Peters E, Harris J. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry. Biochemistry. 2004;43:6535-44 pubmed
Dang L, Melandri F, Stein R. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry. 1998;37:1868-79 pubmed
product information
Product ID :
BML-SE211
Catalog Number :
BML-SE211-0025
Product Name :
Ubiquitin-AMC
Product Type :
Protein
Size :
25 µg
list of Pubmed id :
15157086, 9485312
Product Description :
Ubiquitin-AMC is a fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). It is a particularly useful reagent for the study of deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km = 0.039μM) (Prod. No. BML-UW9745) and for Isopeptidase-T (Km = 0.17-1.4μM) (Prod. No. BML-UW9690), and is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.
Purity :
≥95% (HPLC)
Buffer :
In DMSO
Uses :
Can be used for the following applications:. 1. Substrate for deubiquitinylating enzyme activity assays. 2. Identification/confirmation of enzyme deubiquitinylation activity. 3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL substrates.
Storage :
-80°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.