Proteinase-activated receptor-4 (extracellular) polyclonal antibody | Enzo Life Sciences BML-SA652-0050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteinase-activated receptor-4 (extracellular) polyclonal antibody

catalog :

BML-SA652-0050

quantity :

50 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

Product ID :

BML-SA652

Catalog Number :

BML-SA652-0050

Product Name :

Proteinase-activated receptor-4 (extracellular) polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

50 µl

SPECIES REACTIVITY :

Human, Rat

Background :

PAR-4 belongs to a four member family of G protein-coupled receptors (PAR-1 to -4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this modality of activation, a specific proteinase cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This cleavage results in the creation of a new N-terminal sequence (tethered ligand), which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways1-3 Different PARs are activated by different proteinases. PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated by thrombin and PAR-2 is activated by trypsin1-3. PAR-4 can be also cleaved and activated by other proteinases such as cathepsin G. The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they may involve calcium mobilization downstream of phospholipase C through the Gαq pathway1-3 Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived form PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation.PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell function1-3.

ALTnames :

PAR-4

Immunogen :

Synthetic peptide corresponding to aa 136-150 (with a C149S replacement) of human PAR-4 (proteinase-activated receptor-4).

Application Summary :

FC, ICC, IHC, WB

Storage :

-20°C