PARP-1 monoclonal antibody (C-2-10) | Enzo Life Sciences BML-SA250-0050 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

PARP-1 monoclonal antibody (C-2-10)

catalog :

BML-SA250-0050

quantity :

50 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C-2-10

reactivity :

human, mouse, rat, rhesus macaque

application :

western blot, ELISA, immunohistochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; fig 6	Koyani C, Kitz K, Rossmann C, Bernhart E, Huber E, Trummer C, et al. Activation of the MAPK/Akt/Nrf2-Egr1/HO-1-GCLc axis protects MG-63 osteosarcoma cells against 15d-PGJ2-mediated cell death. Biochem Pharmacol. 2016;104:29-41 pubmed publisher
western blot; human; 1:3000; fig 1a	Rasmussen R, Gajjar M, Jensen K, Hamerlik P. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol. 2016;10:751-63 pubmed publisher
western blot; human; fig 3	Liberante F, Pouryahya T, McMullin M, Zhang S, Mills K. Identification and validation of the dopamine agonist bromocriptine as a novel therapy for high-risk myelodysplastic syndromes and secondary acute myeloid leukemia. Oncotarget. 2016;7:6609-19 pubmed publisher
western blot; human; loading ...; fig 5b	Charni M, Molchadsky A, Goldstein I, Solomon H, Tal P, Goldfinger N, et al. Novel p53 target genes secreted by the liver are involved in non-cell-autonomous regulation. Cell Death Differ. 2016;23:509-20 pubmed publisher
western blot; human	Majuelos Melguizo J, RodrÃguez M, LÃ³pez JimÃ©nez L, RodrÃguez Vargas J, MartÃ MartÃn Consuegra J, Serrano SÃ¡enz S, et al. PARP targeting counteracts gliomagenesis through induction of mitotic catastrophe and aggravation of deficiency in homologous recombination in PTEN-mutant glioma. Oncotarget. 2015;6:4790-803 pubmed
western blot; human; fig 3	Geserick P, Wang J, Feoktistova M, Leverkus M. The ratio of Mcl-1 and Noxa determines ABT737 resistance in squamous cell carcinoma of the skin. Cell Death Dis. 2014;5:e1412 pubmed publisher
western blot; human; fig 3 western blot; mouse	Resch U, Cuapio A, Sturtzel C, Hofer E, de Martin R, Holper Schichl Y. Polyubiquitinated tristetraprolin protects from TNF-induced, caspase-mediated apoptosis. J Biol Chem. 2014;289:25088-100 pubmed publisher
western blot; human; 1:1000	Gastaldello S, Chen X, Callegari S, Masucci M. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog. 2013;9:e1003664 pubmed publisher
western blot; human	Sémiramoth N, Gleizes A, Turbica I, Sandré C, Marin Esteban V, Gorges R, et al. Afa/Dr-expressing, diffusely adhering Escherichia coli strain C1845 triggers F1845 fimbria-dependent phosphatidylserine externalization on neutrophil-like differentiated PLB-985 cells through an apoptosis-independent mechanism. Infect Immun. 2010;78:2974-83 pubmed publisher
western blot; human	Koschny R, Holland H, Sykora J, Erdal H, Krupp W, Bauer M, et al. Bortezomib sensitizes primary human esthesioneuroblastoma cells to TRAIL-induced apoptosis. J Neurooncol. 2010;97:171-85 pubmed publisher
western blot; human	Rossi M, Carbone M, Mostocotto C, Mancone C, Tripodi M, Maione R, et al. Mitochondrial localization of PARP-1 requires interaction with mitofilin and is involved in the maintenance of mitochondrial DNA integrity. J Biol Chem. 2009;284:31616-24 pubmed publisher
western blot; human	Koschny R, Ganten T, Sykora J, Haas T, Sprick M, Kolb A, et al. TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window. Hepatology. 2007;45:649-58 pubmed
western blot; rhesus macaque	Martin Latil S, Mousson L, Autret A, Colbere Garapin F, Blondel B. Bax is activated during rotavirus-induced apoptosis through the mitochondrial pathway. J Virol. 2007;81:4457-64 pubmed
	Zelensky A, Schimmel J, Kool H, Kanaar R, Tijsterman M. Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA. Nat Commun. 2017;8:66 pubmed publisher
	Collins T, Ylanko J, Geng F, Andrews D. A Versatile Cell Death Screening Assay Using Dye-Stained Cells and Multivariate Image Analysis. Assay Drug Dev Technol. 2015;13:547-57 pubmed publisher
	Ciccarone F, Valentini E, Zampieri M, Caiafa P. 5mC-hydroxylase activity is influenced by the PARylation of TET1 enzyme. Oncotarget. 2015;6:24333-47 pubmed
	Knorr K, Schneider P, Meng X, Dai H, Smith B, Hess A, et al. MLN4924 induces Noxa upregulation in acute myelogenous leukemia and synergizes with Bcl-2 inhibitors. Cell Death Differ. 2015;22:2133-42 pubmed publisher
	Xu H, Cho S, Bang M, Bae C, Choi Y, Li Y, et al. FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-ÎºB phosphorylation and COX-2 expression. Int J Oncol. 2015;46:2309-16 pubmed publisher
	Greenhough A, Wallam C, Hicks D, Moorghen M, Williams A, Paraskeva C. The proapoptotic BH3-only protein Bim is downregulated in a subset of colorectal cancers and is repressed by antiapoptotic COX-2/PGE(2) signalling in colorectal adenoma cells. Oncogene. 2010;29:3398-410 pubmed publisher
	Schmidt M, Hupe M, Endres N, Raghavan B, Kavuri S, Geserick P, et al. The contact allergen nickel sensitizes primary human endothelial cells and keratinocytes to TRAIL-mediated apoptosis. J Cell Mol Med. 2010;14:1760-76 pubmed publisher
	Inbal B, Bialik S, Sabanay I, Shani G, Kimchi A. DAP kinase and DRP-1 mediate membrane blebbing and the formation of autophagic vesicles during programmed cell death. J Cell Biol. 2002;157:455-68 pubmed

product information

Product ID :

BML-SA250

Catalog Number :

BML-SA250-0050

Product Name :

PARP-1 monoclonal antibody (C-2-10)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µl

list of Pubmed id :

26358154, 25823424

Clone :

C-2-10

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Monkey, Hamster, Bovine

ALTnames :

Poly(ADP-ribose) polymerase-1

Immunogen :

Purified calf thymus poly(ADP-ribose) polymerase (PARP).

Specificity :

Recognizes an epitope in the C-terminal part of the DNA binding domain of PARP.

Buffer :

Liquid. Mouse ascites containing 0.02% sodium azide.

Uses :

Detects bands ~116kDa (intact PARP) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot.

Application Summary :

ELISA, IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage