PARP-1 monoclonal antibody (C-2-10) (affinity purified) | Enzo Life Sciences BML-SA249-0050 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

PARP-1 monoclonal antibody (C-2-10) (affinity purified)

catalog :

BML-SA249-0050

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C-2-10

The same clone is also sold as:

MilliporeSigma: P248
Invitrogen: MA3-950
BD Biosciences: 556362
Santa Cruz Biotechnology: sc-53643

reactivity :

human, mouse, rat, bovine, rhesus macaque

application :

western blot, ELISA, immunocytochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 2	Hey F, Giblett S, Forrest S, Herbert C, Pritchard C. Phosphorylations of Serines 21/9 in Glycogen Synthase Kinase 3α/β Are Not Required for Cell Lineage Commitment or WNT Signaling in the Normal Mouse Intestine. PLoS ONE. 2016;11:e0156877 pubmed publisher
western blot; human; fig 3	Liberante F, Pouryahya T, McMullin M, Zhang S, Mills K. Identification and validation of the dopamine agonist bromocriptine as a novel therapy for high-risk myelodysplastic syndromes and secondary acute myeloid leukemia. Oncotarget. 2016;7:6609-19 pubmed publisher
western blot; human; loading ...; fig 5b	Charni M, Molchadsky A, Goldstein I, Solomon H, Tal P, Goldfinger N, et al. Novel p53 target genes secreted by the liver are involved in non-cell-autonomous regulation. Cell Death Differ. 2016;23:509-20 pubmed publisher
western blot; human	Majuelos Melguizo J, RodrÃguez M, LÃ³pez JimÃ©nez L, RodrÃguez Vargas J, MartÃ MartÃn Consuegra J, Serrano SÃ¡enz S, et al. PARP targeting counteracts gliomagenesis through induction of mitotic catastrophe and aggravation of deficiency in homologous recombination in PTEN-mutant glioma. Oncotarget. 2015;6:4790-803 pubmed
western blot; human; fig 3	Geserick P, Wang J, Feoktistova M, Leverkus M. The ratio of Mcl-1 and Noxa determines ABT737 resistance in squamous cell carcinoma of the skin. Cell Death Dis. 2014;5:e1412 pubmed publisher
western blot; human	Sémiramoth N, Gleizes A, Turbica I, Sandré C, Marin Esteban V, Gorges R, et al. Afa/Dr-expressing, diffusely adhering Escherichia coli strain C1845 triggers F1845 fimbria-dependent phosphatidylserine externalization on neutrophil-like differentiated PLB-985 cells through an apoptosis-independent mechanism. Infect Immun. 2010;78:2974-83 pubmed publisher
western blot; human	Koschny R, Holland H, Sykora J, Erdal H, Krupp W, Bauer M, et al. Bortezomib sensitizes primary human esthesioneuroblastoma cells to TRAIL-induced apoptosis. J Neurooncol. 2010;97:171-85 pubmed publisher
western blot; human	Rossi M, Carbone M, Mostocotto C, Mancone C, Tripodi M, Maione R, et al. Mitochondrial localization of PARP-1 requires interaction with mitofilin and is involved in the maintenance of mitochondrial DNA integrity. J Biol Chem. 2009;284:31616-24 pubmed publisher
western blot; human	Koschny R, Ganten T, Sykora J, Haas T, Sprick M, Kolb A, et al. TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window. Hepatology. 2007;45:649-58 pubmed
western blot; rhesus macaque	Martin Latil S, Mousson L, Autret A, Colbere Garapin F, Blondel B. Bax is activated during rotavirus-induced apoptosis through the mitochondrial pathway. J Virol. 2007;81:4457-64 pubmed
	Zelensky A, Schimmel J, Kool H, Kanaar R, Tijsterman M. Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA. Nat Commun. 2017;8:66 pubmed publisher
	Grellety T, Laroche Clary A, Chaire V, Lagarde P, Chibon F, Neuville A, et al. PRIMA-1(MET) induces death in soft-tissue sarcomas cell independent of p53. BMC Cancer. 2015;15:684 pubmed publisher
	Collins T, Ylanko J, Geng F, Andrews D. A Versatile Cell Death Screening Assay Using Dye-Stained Cells and Multivariate Image Analysis. Assay Drug Dev Technol. 2015;13:547-57 pubmed publisher
	Ciccarone F, Valentini E, Zampieri M, Caiafa P. 5mC-hydroxylase activity is influenced by the PARylation of TET1 enzyme. Oncotarget. 2015;6:24333-47 pubmed
	Knorr K, Schneider P, Meng X, Dai H, Smith B, Hess A, et al. MLN4924 induces Noxa upregulation in acute myelogenous leukemia and synergizes with Bcl-2 inhibitors. Cell Death Differ. 2015;22:2133-42 pubmed publisher
	Jacob K, Noren Hooten N, Tadokoro T, Lohani A, Barnes J, Evans M. Alzheimer's disease-associated polymorphisms in human OGG1 alter catalytic activity and sensitize cells to DNA damage. Free Radic Biol Med. 2013;63:115-25 pubmed publisher
	Greenhough A, Wallam C, Hicks D, Moorghen M, Williams A, Paraskeva C. The proapoptotic BH3-only protein Bim is downregulated in a subset of colorectal cancers and is repressed by antiapoptotic COX-2/PGE(2) signalling in colorectal adenoma cells. Oncogene. 2010;29:3398-410 pubmed publisher
	Schmidt M, Hupe M, Endres N, Raghavan B, Kavuri S, Geserick P, et al. The contact allergen nickel sensitizes primary human endothelial cells and keratinocytes to TRAIL-mediated apoptosis. J Cell Mol Med. 2010;14:1760-76 pubmed publisher
	Lepretre C, Scovassi A, Shah G, Torriglia A. Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis. Int J Biochem Cell Biol. 2009;41:1046-54 pubmed publisher
	Guastafierro T, Cecchinelli B, Zampieri M, Reale A, Riggio G, Sthandier O, et al. CCCTC-binding factor activates PARP-1 affecting DNA methylation machinery. J Biol Chem. 2008;283:21873-80 pubmed publisher
	Cipriani G, Rapizzi E, Vannacci A, Rizzuto R, Moroni F, Chiarugi A. Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction. J Biol Chem. 2005;280:17227-34 pubmed
	Javelaud D, Besancon F. Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio. J Biol Chem. 2002;277:37949-54 pubmed
	Inbal B, Bialik S, Sabanay I, Shani G, Kimchi A. DAP kinase and DRP-1 mediate membrane blebbing and the formation of autophagic vesicles during programmed cell death. J Cell Biol. 2002;157:455-68 pubmed
	Gobeil S, Boucher C, Nadeau D, Poirier G. Characterization of the necrotic cleavage of poly(ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases. Cell Death Differ. 2001;8:588-94 pubmed
	Alvarez Gonzalez R, Spring H, Muller M, Burkle A. Selective loss of poly(ADP-ribose) and the 85-kDa fragment of poly(ADP-ribose) polymerase in nucleoli during alkylation-induced apoptosis of HeLa cells. J Biol Chem. 1999;274:32122-6 pubmed
	Duriez P, Desnoyers S, Hoflack J, Shah G, Morelle B, Bourassa S, et al. Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase. Biochim Biophys Acta. 1997;1334:65-72 pubmed
	Datta R, Kojima H, Banach D, Bump N, Talanian R, Alnemri E, et al. Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis. J Biol Chem. 1997;272:1965-9 pubmed

product information

Product ID :

BML-SA249

Catalog Number :

BML-SA249-0050

Product Name :

PARP-1 monoclonal antibody (C-2-10) (affinity purified)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

26463477, 23684897, 18951996, 18539602, 15750180, 12151395, 11536009, 10542247, 9042367, 8999887

Clone :

C-2-10

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Monkey, Hamster, Bovine

ALTnames :

Poly(ADP-ribose) polymerase-1

Immunogen :

Purified calf thymus poly(ADP-ribose) polymerase (PARP).

Specificity :

Recognizes an epitope in the C-terminal part of the DNA binding domain of PARP.

Buffer :

Liquid. In PBS containing 0.02% sodium azide.

Uses :

Detects bands ~116kDa (intact PARP) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot.

Application Summary :

ELISA, ICC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage