p62 (human) polyclonal antibody | Enzo Life Sciences BML-PW9860-0025 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

p62 (human) polyclonal antibody

catalog :

BML-PW9860-0025

quantity :

25 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; loading ...; fig 4b	Silva M, Nandi G, Tentarelli S, Gurrell I, Jamier T, Lucente D, et al. Prolonged tau clearance and stress vulnerability rescue by pharmacological activation of autophagy in tauopathy neurons. Nat Commun. 2020;11:3258 pubmed publisher
immunocytochemistry; human; 1:200; loading ...; fig 4a western blot; human; 1:250; loading ...; fig 4b	De Luna N, Turon Sans J, Cortés Vicente E, Carrasco Rozas A, Illán Gala I, Dols Icardo O, et al. Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis. Sci Rep. 2020;10:4308 pubmed publisher
immunoprecipitation; human; loading ...; fig 5f immunocytochemistry; human; 1:200; loading ...; fig 5h western blot; human; 1:1000; loading ...; fig 5i	Iurlaro R, Püschel F, León Annicchiarico C, O Connor H, Martin S, Palou Gramón D, et al. Glucose Deprivation Induces ATF4-Mediated Apoptosis through TRAIL Death Receptors. Mol Cell Biol. 2017;37: pubmed publisher
western blot; human; fig 2	Relic B, Charlier E, Deroyer C, Malaise O, Neuville S, Desoroux A, et al. BAY 11-7085 induces glucocorticoid receptor activation and autophagy that collaborate with apoptosis to induce human synovial fibroblast cell death. Oncotarget. 2016;7:23370-82 pubmed publisher
western blot; mouse; fig 2	Li W, Zou J, Yue F, Song K, Chen Q, McKeehan W, et al. Defects in MAP1S-mediated autophagy cause reduction in mouse lifespans especially when fibronectin is overexpressed. Aging Cell. 2016;15:370-9 pubmed publisher
western blot; mouse; 1:1000; fig 2	Xie C, Ginet V, Sun Y, Koike M, Zhou K, Li T, et al. Neuroprotection by selective neuronal deletion of Atg7 in neonatal brain injury. Autophagy. 2016;12:410-23 pubmed publisher
	Blazquez C, Ruiz Calvo A, Bajo Grañeras R, Baufreton J, Resel E, Varilh M, et al. Inhibition of striatonigral autophagy as a link between cannabinoid intoxication and impairment of motor coordination. elife. 2020;9: pubmed publisher

product information

Product ID :

BML-PW9860

Catalog Number :

BML-PW9860-0025

Product Name :

p62 (human) polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

25 µl

list of Pubmed id :

26363065, 26244336, 25880015, 25583186, 25158237, 24722279, 24275666, 24211199, 23960084, 23916833

SPECIES REACTIVITY :

Human

Background :

P62, also known as Sequestosome I, is a 62kDa, 440 amino acid protein, initially identified as a ligand of the SH2 domain of p56lck, now known to be expressed in many tissues. In addition to TRAF6, PEST and zinc finger motifs, p62 has a C-terminal ubiquitin binding association (UBA) domain with an affinity for multi-ubiquitin chains, and it is considered to serve as a scaffold protein, capable of binding to multiple signalling molecules and uniting receptor-mediated signalling events with ubiquitinylation. Elevated levels of p62 have been reported in breast tumours and in alcoholic liver disease where p62 has been shown to be involved in the formation of Mallory bodies. Several mutations in the p62 UBA domain have been identified and the etiology of Paget’s disease of bone has been linked to one such mutation. Kuusisto and colleagues have demonstrated that p62 is also present in elevated levels in the hallmark inclusions found in various neurodegenerative conditions, including tauopathies (Alzheimer’s disease, Picks disease, and frontotemporal dementia) and synucleinopathies (Parkinson’s disease, dementia with Lewy body disease and multiple system atrophy). In recent years ubiquitin immunostaining has been used to provide adjunct information for neuropathological diagnosis, but it is becoming evident that p62 may be an even more reliable marker of neurodegenerative disease inclusion detection than tau, alpha-synuclein or ubiquitin immunostaining.

ALTnames :

SQSTM1, Sequestosome I

Immunogen :

Synthetic peptide corresponding to aa 387-436 human p62.

Buffer :

Liquid. Contains 0.1% sodium azide.

Application Summary :

ELISA, IF, IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage