Proteasome 20S β2 subunit monoclonal antibody (MCP165) | Enzo Life Sciences BML-PW9300-0025 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 20S β2 subunit monoclonal antibody (MCP165)

catalog :

BML-PW9300-0025

quantity :

25 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MCP165

The same clone is also sold as:

Abcam: ab22650
Santa Cruz Biotechnology: sc-58410

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Golnik R, Lehmann A, Kloetzel P, Ebstein F. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination. J Biol Chem. 2016;291:8805-15 pubmed publisher
western blot; human; 1:1500; fig 5	Balsas P, Galán Malo P, Marzo I, Naval J. Bortezomib resistance in a myeloma cell line is associated to PSM?5 overexpression and polyploidy. Leuk Res. 2012;36:212-8 pubmed publisher
	Poli M, Ebstein F, Nicholas S, de Guzman M, Forbes L, Chinn I, et al. Heterozygous Truncating Variants in POMP Escape Nonsense-Mediated Decay and Cause a Unique Immune Dysregulatory Syndrome. Am J Hum Genet. 2018;102:1126-1142 pubmed publisher
	Homma T, Kurahashi T, Lee J, Kang E, Fujii J. SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes. Arch Biochem Biophys. 2015;583:65-72 pubmed publisher

product information

Product ID :

BML-PW9300

Catalog Number :

BML-PW9300-0025

Product Name :

Proteasome 20S β2 subunit monoclonal antibody (MCP165)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

25 µl

list of Pubmed id :

26264915

Clone :

MCP165

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse

Background :

The hybridoma secreting the antibody to subunit β2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with dinitrophenylated proteasomes. The antibody (clone MCP165) has been characterised by one- and two dimensional Western blotting. The antibody has not been demonstrated to immunoprecipitate the native 20S proteasome. The immunoglobulin subclass is IgG1.

ALTnames :

Proteasome subunit β type-7, Macropain chain Z

Immunogen :

Dinitrophenylated proteasomes.

Specificity :

Recognizes the β2 subunit of the 20S proteasome.

Buffer :

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Uses :

Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations. Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue. Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa.

Application Summary :

IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage