Proteasome 19S ATPase subunit Rpt6 monoclonal antibody (p45-110) | Enzo Life Sciences BML-PW9265-0025 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 19S ATPase subunit Rpt6 monoclonal antibody (p45-110)

catalog :

BML-PW9265-0025

quantity :

25 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

p45-110

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; fig 3	Vingill S, Brockelt D, Lancelin C, Tatenhorst L, Dontcheva G, Preisinger C, et al. Loss of FBXO7 (PARK15) results in reduced proteasome activity and models a parkinsonism-like phenotype in mice. EMBO J. 2016;35:2008-25 pubmed publisher
western blot; mouse; fig 2 western blot; human; fig 1	Hjerpe R, Bett J, Keuss M, Solovyova A, McWilliams T, Johnson C, et al. UBQLN2 Mediates Autophagy-Independent Protein Aggregate Clearance by the Proteasome. Cell. 2016;166:935-949 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3a	Welk V, Coux O, Kleene V, Abeza C, Trumbach D, Eickelberg O, et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem. 2016;291:13147-59 pubmed publisher
western blot; rat; 1:10	Farley M, Swulius M, Waxham M. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience. 2015;304:286-301 pubmed publisher
western blot; human; fig 3	Bollinger L, Powell J, Houmard J, Witczak C, Brault J. Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux. Obesity (Silver Spring). 2015;23:1185-93 pubmed publisher
western blot; rat; 1:2000	Huang Q, Wang H, Perry S, Figueiredo Pereira M. Negative regulation of 26S proteasome stability via calpain-mediated cleavage of Rpn10 subunit upon mitochondrial dysfunction in neurons. J Biol Chem. 2013;288:12161-74 pubmed publisher
	Poli M, Ebstein F, Nicholas S, de Guzman M, Forbes L, Chinn I, et al. Heterozygous Truncating Variants in POMP Escape Nonsense-Mediated Decay and Cause a Unique Immune Dysregulatory Syndrome. Am J Hum Genet. 2018;102:1126-1142 pubmed publisher
	Santiago Sim T, Burrage L, Ebstein F, Tokita M, Miller M, Bi W, et al. Biallelic Variants in OTUD6B Cause an Intellectual Disability Syndrome Associated with Seizures and Dysmorphic Features. Am J Hum Genet. 2017;100:676-688 pubmed publisher
	Myeku N, Figueiredo Pereira M. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 2011;286:22426-40 pubmed publisher
	Hendil K, Kriegenburg F, Tanaka K, Murata S, Lauridsen A, Johnsen A, et al. The 20S proteasome as an assembly platform for the 19S regulatory complex. J Mol Biol. 2009;394:320-8 pubmed publisher

product information

Product ID :

BML-PW9265

Catalog Number :

BML-PW9265-0025

Product Name :

Proteasome 19S ATPase subunit Rpt6 monoclonal antibody (p45-110)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

25 µl

list of Pubmed id :

26215919, 26010327, 21536669

Clone :

p45-110

Isotype :

IgG2b

SPECIES REACTIVITY :

Human, Mouse, Rat

Background :

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha-subunits comprise the outer rings and the beta-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha7-beta7-beta7-alpha7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the alpha- or beta-rings . In addition to the 20S particle, the 26S complex contains over twenty additional proteins, ranging in molecular weight from 25 to 10kDa, located in a distinct complex called the ‘PA700 proteasome activator’ or the ‘19S complex’, and which determines substrate specificity and provides the multiple enzymatic functions necessary for proteolysis and viability. Systematic analysis of the sub-unit components have revealed at least six members to be ATPases belonging to a new family of ATPbinding proteins, together with a further fifteen sub-units that lack the capacity to bind ATP, isopeptidases and several other proteins thought to be responsible for the unfolding of a protein substrate prior to insertion into the proteolytic core of the 20S proteasome.

ALTnames :

26S protease regulatory subunit 8, Proteasome 26S subunit ATPase 5, TRIP1, Thyroid hormone receptor-interacting protein 1, p45/SUG

Immunogen :

Recombinant human Rpt6 protein.

Specificity :

Recognizes the Rpt6/S subunit of the 19S regulator complex.

Buffer :

Liquid. In PBS, pH 7.4, containing 10mM sodium azide.

Uses :

Detects a band of ~48kDa by Western blot.

Application Summary :

IHC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage