Proteasome 20S β5i subunit monoclonal antibody (LMP7-1) | Enzo Life Sciences BML-PW8845-0025 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 20S β5i subunit monoclonal antibody (LMP7-1)

catalog :

BML-PW8845-0025

quantity :

25 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LMP7-1

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Enzo Life Sciences product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; fig 5a	Chang A, Dao T, Gejman R, Jarvis C, Scott A, Dubrovsky L, et al. A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens. J Clin Invest. 2017;127:2705-2718 pubmed publisher
immunohistochemistry - paraffin section; human; 1:5000; loading ...; fig 1d, 5a	Kasacka I, Piotrowska Z, Niezgoda M, Lewandowska A, Łebkowski W. Ageing-related changes in the levels of β-catenin, CacyBP/SIP, galectin-3 and immunoproteasome subunit LMP7 in the heart of men. PLoS ONE. 2020;15:e0229462 pubmed publisher
immunohistochemistry - paraffin section; human; 1:700 western blot; human; 1:1000	Vasuri F, Capizzi E, Bellavista E, Mishto M, Santoro A, Fiorentino M, et al. Studies on immunoproteasome in human liver. Part I: absence in fetuses, presence in normal subjects, and increased levels in chronic active hepatitis and cirrhosis. Biochem Biophys Res Commun. 2010;397:301-6 pubmed publisher
	Mishto M, Raza M, de Biase D, Ravizza T, Vasuri F, Martucci M, et al. The immunoproteasome Î²5i subunit is a key contributor to ictogenesis in a rat model of chronic epilepsy. Brain Behav Immun. 2015;49:188-96 pubmed publisher
	Bergamo P, Palmieri G, Cocca E, Ferrandino I, Gogliettino M, Monaco A, et al. Adaptive response activated by dietary cis9, trans11 conjugated linoleic acid prevents distinct signs of gliadin-induced enteropathy in mice. Eur J Nutr. 2016;55:729-740 pubmed publisher
	Dechavanne V, Vilbois F, Glez L, Antonsson B. Purification and separation of the 20S immunoproteasome from the constitutive proteasome and identification of the subunits by LC-MS. Protein Expr Purif. 2013;87:100-10 pubmed publisher
	Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, et al. Immunoproteasome expression is induced in mesial temporal lobe epilepsy. Biochem Biophys Res Commun. 2011;408:65-70 pubmed publisher
	Bianchi G, Oliva L, Cascio P, Pengo N, Fontana F, Cerruti F, et al. The proteasome load versus capacity balance determines apoptotic sensitivity of multiple myeloma cells to proteasome inhibition. Blood. 2009;113:3040-9 pubmed publisher

product information

Product ID :

BML-PW8845

Catalog Number :

BML-PW8845-0025

Product Name :

Proteasome 20S β5i subunit monoclonal antibody (LMP7-1)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

25 µl

list of Pubmed id :

26044087, 23147206, 21458417, 20510670, 19164601

Clone :

LMP7-1

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Rat

Background :

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. Lmp2, Lmp7 and MECL are interferon gamma-inducible catalytic subunits of the 20S proteasome which may replace the constitutive catalytic subunits, delta, X and Z respectively, during proteasome biogenesis. Lmp2 and Lmp7 alter the cleavage site specificity of the 20S proteasome and are required for the efficient generation of T cell epitopes from a number of viral proteins and for optimal MHC class I cell surface expression .

ALTnames :

Proteasome subunit β type-8, LMP7, RING10 protein, Macropain subunit C13

Immunogen :

Recombinant human proteasome subunit β5i.

Specificity :

Recognizes the β5i subunit of proteasome 20S.

Buffer :

Liquid. In PBS, pH 7.4, containing 10mM sodium azide.

Uses :

The serum was characterised by single dimension SDS-PAGE using a HeLa cell lysate both with and without prior stimulation with γ-interferon. The mature LMP7 protein giving a band at a relative molecular weight of ~25kDa was detected by Western blotting in both stimulated and nonstimulated cells. Not suitable for immunoprecipitation.

Application Summary :

IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage