Proteasome 20S β1i subunit monoclonal antibody (LMP2 - 13) | Enzo Life Sciences BML-PW8840-0025 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 20S β1i subunit monoclonal antibody (LMP2 - 13)

catalog :

BML-PW8840-0025

quantity :

25 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LMP2-13

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Enzo Life Sciences product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; fig 5a	Chang A, Dao T, Gejman R, Jarvis C, Scott A, Dubrovsky L, et al. A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens. J Clin Invest. 2017;127:2705-2718 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200 western blot; human; 1:1000	Vasuri F, Capizzi E, Bellavista E, Mishto M, Santoro A, Fiorentino M, et al. Studies on immunoproteasome in human liver. Part I: absence in fetuses, presence in normal subjects, and increased levels in chronic active hepatitis and cirrhosis. Biochem Biophys Res Commun. 2010;397:301-6 pubmed publisher
	Martinez Gamboa L, Lesemann K, Kuckelkorn U, Scheffler S, Ghannam K, Hahne M, et al. Gene expression of catalytic proteasome subunits and resistance toward proteasome inhibition of B lymphocytes from patients with primary sjogren syndrome. J Rheumatol. 2013;40:663-73 pubmed publisher
	Dechavanne V, Vilbois F, Glez L, Antonsson B. Purification and separation of the 20S immunoproteasome from the constitutive proteasome and identification of the subunits by LC-MS. Protein Expr Purif. 2013;87:100-10 pubmed publisher
	Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, et al. Immunoproteasome expression is induced in mesial temporal lobe epilepsy. Biochem Biophys Res Commun. 2011;408:65-70 pubmed publisher
	Bianchi G, Oliva L, Cascio P, Pengo N, Fontana F, Cerruti F, et al. The proteasome load versus capacity balance determines apoptotic sensitivity of multiple myeloma cells to proteasome inhibition. Blood. 2009;113:3040-9 pubmed publisher

product information

Product ID :

BML-PW8840

Catalog Number :

BML-PW8840-0025

Product Name :

Proteasome 20S β1i subunit monoclonal antibody (LMP2 - 13)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

25 µl

list of Pubmed id :

23504381, 23147206, 21458417, 20510670, 19164601

Clone :

LMP2-13

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Rat

Background :

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. Lmp2, Lmp7 and MECL are interferon gamma-inducible catalytic subunits of the 20S proteasome which may replace the constitutive catalytic subunits, delta, X and Z respectively, during proteasome biogenesis. Lmp2 and Lmp7 alter the cleavage site specificity of the 20S proteasome and are required for the efficient generation of T cell epitopes from a number of viral proteins and for optimal MHC class I cell surface expression.

ALTnames :

Proteasome subunit β type-9, RING12 protein, LMP2

Immunogen :

Recombinant human β1i protein.

Specificity :

Recognizes the β1i subunit of proteasome 20S.

Buffer :

Liquid. In PBS containing 10mM sodium azide.

Uses :

The serum was characterised by single dimension SDS-PAGE using a HeLa cell lysate both with and without prior stimulation with γ-interferon. The mature LMP2 protein of ~22kDa was detected by Western blotting in the γ-interferon stimulated cells and additionally the precursor form at ~25kDa. Not suitable for immunoprecipitation.

Application Summary :

IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage