| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot; human; loading ...; fig s16h
| Bigenzahn J, Collu G, Kartnig F, Pieraks M, Vladimer G, Heinz L, et al. LZTR1 is a regulator of RAS ubiquitination and signaling. Science. 2018;362:1171-1177 pubmed publisher |
- immunocytochemistry; human; 1:200; loading ...; fig s5f
| Rai A, Chen J, Selbach M, Pelkmans L. Kinase-controlled phase transition of membraneless organelles in mitosis. Nature. 2018;559:211-216 pubmed publisher |
- immunohistochemistry; human; loading ...; fig 2b
| Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, et al. p62 filaments capture and present ubiquitinated cargos for autophagy. EMBO J. 2018;37: pubmed publisher |
- immunohistochemistry; fruit fly ; loading ...; fig 1h
| Anding A, Wang C, Chang T, Sliter D, Powers C, Hofmann K, et al. Vps13D Encodes a Ubiquitin-Binding Protein that Is Required for the Regulation of Mitochondrial Size and Clearance. Curr Biol. 2018;28:287-295.e6 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 3e
| Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed publisher |
- immunohistochemistry; mouse; fig 4g
| Mendoza A, Fang V, Chen C, Serasinghe M, Verma A, Muller J, et al. Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells. Nature. 2017;546:158-161 pubmed publisher |
- immunocytochemistry; human; loading ...; fig 4a
| Paul A, Wang B. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage. Mol Cell. 2017;66:458-472.e5 pubmed publisher |
- western blot; fruit fly ; loading ...; fig 4e
| Vonk J, Yeshaw W, Pinto F, Faber A, Lahaye L, Kanon B, et al. Drosophila Vps13 Is Required for Protein Homeostasis in the Brain. PLoS ONE. 2017;12:e0170106 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig ED8g
| Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;541:541-545 pubmed publisher |
- western blot; human; loading ...; fig 5a
| Steffen J, Vashisht A, Wan J, Jen J, Claypool S, Wohlschlegel J, et al. Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria. Mol Biol Cell. 2017;28:600-612 pubmed publisher |
- western blot; human; loading ...; fig s3b
| Ginster S, Bardet M, Unterreiner A, Malinverni C, Renner F, Lam S, et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS ONE. 2017;12:e0169026 pubmed publisher |
- immunocytochemistry; mouse; loading ...; fig 4b
| Abshire C, Dragoi A, Roy C, Ivanov S. MTOR-Driven Metabolic Reprogramming Regulates Legionella pneumophila Intracellular Niche Homeostasis. PLoS Pathog. 2016;12:e1006088 pubmed publisher |
- immunocytochemistry; mouse; fig 8
| Dragich J, Kuwajima T, Hirose Ikeda M, Yoon M, Eenjes E, Bosco J, et al. Autophagy linked FYVE (Alfy/WDFY3) is required for establishing neuronal connectivity in the mammalian brain. elife. 2016;5: pubmed publisher |
- western blot; mouse; 1:2000; loading ...; fig 7d
- western blot; human; 1:2000; loading ...; fig 7c
| Guturi K, Bohgaki M, Bohgaki T, Srikumar T, Ng D, Kumareswaran R, et al. RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation. Nat Commun. 2016;7:12638 pubmed publisher |
- western blot; mouse; loading ...; fig 6a
| Reeg S, Jung T, Castro J, Davies K, Henze A, Grune T. The molecular chaperone Hsp70 promotes the proteolytic removal of oxidatively damaged proteins by the proteasome. Free Radic Biol Med. 2016;99:153-166 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; loading ...; fig 4a
| Walker W, Oehler A, Edinger A, Wagner K, Gunn T. Oligodendroglial deletion of ESCRT-I component TSG101 causes spongiform encephalopathy. Biol Cell. 2016;108:324-337 pubmed publisher |
- western blot; human; 1:400; fig s4
| Liang C, Li Z, Lopez Martinez D, Nicholson W, Venien Bryan C, Cohn M. The FANCD2-FANCI complex is recruited to DNA interstrand crosslinks before monoubiquitination of FANCD2. Nat Commun. 2016;7:12124 pubmed publisher |
- immunohistochemistry - paraffin section; human; fig 7
- western blot; human; fig 5
| Kobayashi K, Araya J, Minagawa S, Hara H, Saito N, Kadota T, et al. Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis. J Immunol. 2016;197:504-16 pubmed publisher |
- immunocytochemistry; human; 1:100; fig s4
| Jullien D, Vignard J, Fedor Y, Bery N, Olichon A, Crozatier M, et al. Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells. J Cell Sci. 2016;129:2673-83 pubmed publisher |
- western blot; human; fig 2
| Richter B, Sliter D, Herhaus L, Stolz A, Wang C, Beli P, et al. Phosphorylation of OPTN by TBK1 enhances its binding to Ub chains and promotes selective autophagy of damaged mitochondria. Proc Natl Acad Sci U S A. 2016;113:4039-44 pubmed publisher |
- western blot; human; 1:2500; fig s11
| Choi W, de Poot S, Lee J, Kim J, Han D, Kim Y, et al. Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation. Nat Commun. 2016;7:10963 pubmed publisher |
- western blot; human; fig 6
| Golnik R, Lehmann A, Kloetzel P, Ebstein F. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination. J Biol Chem. 2016;291:8805-15 pubmed publisher |
- western blot; rat; 1:1000; loading ...; fig 3d
| Liu Z, Sneve M, Haroldson T, Smith J, Drewes L. Regulation of Monocarboxylic Acid Transporter 1 Trafficking by the Canonical Wnt/β-Catenin Pathway in Rat Brain Endothelial Cells Requires Cross-talk with Notch Signaling. J Biol Chem. 2016;291:8059-69 pubmed publisher |
- other; human; loading ...; fig st1
| Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher |
- western blot; mouse; fig 1c
| Yang Y, Li W, Hoque M, Hou L, Shen S, Tian B, et al. PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation. PLoS Genet. 2016;12:e1005794 pubmed publisher |
- western blot; human; fig 7a
| Pedersen S, Chan W, Jattani R, Mackie d, Pomerantz J. Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181. Mol Cell Biol. 2015;36:794-808 pubmed publisher |
- immunohistochemistry - paraffin section; fruit fly ; 1:100; fig s5b
| Gogendeau D, Siudeja K, Gambarotto D, Pennetier C, Bardin A, Basto R. Aneuploidy causes premature differentiation of neural and intestinal stem cells. Nat Commun. 2015;6:8894 pubmed publisher |
- western blot; rat; 1:1000; fig 4
| Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed publisher |
| Briz V, Liu Y, Zhu G, Bi X, Baudry M. A novel form of synaptic plasticity in field CA3 of hippocampus requires GPER1 activation and BDNF release. J Cell Biol. 2015;210:1225-37 pubmed publisher |
- western blot; human; fig 3
| Pitcher D, de Mattos Shipley K, Tzortzis K, Auner H, Karadimitris A, Kleijnen M. Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure. EBioMedicine. 2015;2:642-8 pubmed publisher |
| Parkinson M, Piper S, Bright N, Evans J, Boname J, Bowers K, et al. A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. Biochem J. 2015;471:79-88 pubmed publisher |
- western blot; human; fig 1a
| Kharat S, Tripathi V, Damodaran A, Priyadarshini R, Chandra S, Tikoo S, et al. Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability. Oncogene. 2016;35:1025-38 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 4b
| Chen Y, Peng Y, Hu M, Huang J, Chien Y, Wu J, et al. The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels. Sci Rep. 2015;5:10667 pubmed publisher |
- immunocytochemistry; mouse
| Cemma M, Lam G, Stöckli M, Higgins D, Brumell J. Strain-Specific Interactions of Listeria monocytogenes with the Autophagy System in Host Cells. PLoS ONE. 2015;10:e0125856 pubmed publisher |
- western blot; human; fig s2
| Hotokezaka Y, Katayama I, van Leyen K, Nakamura T. GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses. Cell Death Dis. 2015;6:e1719 pubmed publisher |
| Kocyłowski M, Rey A, Stewart G, Halazonetis T. Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168. Cell Cycle. 2015;14:1748-58 pubmed publisher |
- western blot; human; fig 4
| von Stechow L, Typas D, Carreras Puigvert J, Oort L, Siddappa R, Pines A, et al. The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by initiating a 4EHP-mediated mRNA translation arrest. Mol Cell Biol. 2015;35:1254-68 pubmed publisher |
- western blot; southern flounder; 1:500
| Wilson W, Baumgarner B, Watanabe W, Alam M, Kinsey S. Effects of resveratrol on growth and skeletal muscle physiology of juvenile southern flounder. Comp Biochem Physiol A Mol Integr Physiol. 2015;183:27-35 pubmed publisher |
| Cao M, Milosevic I, Giovedi S, De Camilli P. Upregulation of Parkin in endophilin mutant mice. J Neurosci. 2014;34:16544-9 pubmed publisher |
- immunocytochemistry; rat; 1:400; fig 1
| Leyk J, Goldbaum O, Noack M, Richter Landsberg C. Inhibition of HDAC6 modifies tau inclusion body formation and impairs autophagic clearance. J Mol Neurosci. 2015;55:1031-46 pubmed publisher |
- immunohistochemistry - frozen section; mouse; 1:1000; fig s4
| Gascon E, Lynch K, Ruan H, Almeida S, Verheyden J, Seeley W, et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med. 2014;20:1444-51 pubmed publisher |
- western blot; human; 1:500; loading ...; fig 3a
| Rougier J, Albesa M, Syam N, Halet G, Abriel H, Viard P. Ubiquitin-specific protease USP2-45 acts as a molecular switch to promote α2δ-1-induced downregulation of Cav1.2 channels. Pflugers Arch. 2015;467:1919-29 pubmed publisher |
| Pitcher D, de Mattos Shipley K, Wang Z, Tzortzis K, Goudevenou K, Flynn H, et al. Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE). Biochim Biophys Acta. 2014;1844:2222-8 pubmed publisher |
- chromatin immunoprecipitation; Saccharomycetales
| Bonizec M, Hérissant L, Pokrzywa W, Geng F, Wenzel S, Howard G, et al. The ubiquitin-selective chaperone Cdc48/p97 associates with Ubx3 to modulate monoubiquitylation of histone H2B. Nucleic Acids Res. 2014;42:10975-86 pubmed publisher |
- western blot; human; fig 4
| Baron Y, Pedrioli P, Tyagi K, Johnson C, Wood N, Fountaine D, et al. VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase. BMC Biol. 2014;12:39 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:100; fig 1
- immunocytochemistry; mouse; 1:100
| Zhang L, Chen X, Sharma P, Moon M, Sheftel A, Dawood F, et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat Commun. 2014;5:3430 pubmed publisher |
| McCarthy R, Kosman D. Glial cell ceruloplasmin and hepcidin differentially regulate iron efflux from brain microvascular endothelial cells. PLoS ONE. 2014;9:e89003 pubmed publisher |
- immunoprecipitation; mouse; 1:2000; loading ...; fig 1d
| Calco G, Stephens O, Donahue L, Tsui C, Pierchala B. CD2-associated protein (CD2AP) enhances casitas B lineage lymphoma-3/c (Cbl-3/c)-mediated Ret isoform-specific ubiquitination and degradation via its amino-terminal Src homology 3 domains. J Biol Chem. 2014;289:7307-19 pubmed publisher |
- immunocytochemistry; mouse; 1:500
- western blot; mouse; 1:1000
| Rodriguez Ortiz C, Hoshino H, Cheng D, Liu Yescevitz L, Blurton Jones M, Wolozin B, et al. Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice. Am J Pathol. 2013;183:504-15 pubmed publisher |
- western blot; mouse; 1:5000
| Durieux A, Vassilopoulos S, Laine J, Fraysse B, Brinas L, Prudhon B, et al. A centronuclear myopathy--dynamin 2 mutation impairs autophagy in mice. Traffic. 2012;13:869-79 pubmed publisher |
- immunohistochemistry - paraffin section; human; 1:250
| Paine S, Bedford L, Thorpe J, Mayer R, Cavey J, Bajaj N, et al. Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration. Neurosci Lett. 2009;460:205-8 pubmed publisher |
| Yuan R, Hahn Y, Stempel M, Sidibe D, Laxton O, Chen J, et al. Proteasomal inhibition preferentially stimulates lysosome activity relative to autophagic flux in primary astrocytes. Autophagy. 2022;:1-27 pubmed publisher |
| Bondarieva A, Raveendran K, Telychko V, Rao H, Ravindranathan R, Zorzompokou C, et al. Proline-rich protein PRR19 functions with cyclin-like CNTD1 to promote meiotic crossing over in mouse. Nat Commun. 2020;11:3101 pubmed publisher |
| Zhang R, Varela M, Vallentgoed W, Forn Cuni G, van der Vaart M, Meijer A. The selective autophagy receptors Optineurin and p62 are both required for zebrafish host resistance to mycobacterial infection. PLoS Pathog. 2019;15:e1007329 pubmed publisher |
| Robeson A, Lindblom K, Wojton J, Kornbluth S, Matsuura K. Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators. EMBO J. 2018;37: pubmed publisher |
| Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, et al. Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nat Cell Biol. 2017;19:1400-1411 pubmed publisher |
| Misaka T, Murakawa T, Nishida K, Omori Y, Taneike M, Omiya S, et al. FKBP8 protects the heart from hemodynamic stress by preventing the accumulation of misfolded proteins and endoplasmic reticulum-associated apoptosis in mice. J Mol Cell Cardiol. 2018;114:93-104 pubmed publisher |
| Soh H, Venkatesan N, Veena M, Ravichandran S, Zinabadi A, Basak S, et al. Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-?B. Mol Cell Biol. 2016;36:1776-92 pubmed publisher |
| Speckmann T, Sabatini P, Nian C, Smith R, Lynn F. Npas4 Transcription Factor Expression Is Regulated by Calcium Signaling Pathways and Prevents Tacrolimus-induced Cytotoxicity in Pancreatic Beta Cells. J Biol Chem. 2016;291:2682-95 pubmed publisher |
| Thorslund T, Ripplinger A, Hoffmann S, Wild T, Uckelmann M, Villumsen B, et al. Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage. Nature. 2015;527:389-93 pubmed publisher |
| Johansson I, Monsen V, Pettersen K, Mildenberger J, Misund K, Kaarniranta K, et al. The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells. Autophagy. 2015;11:1636-51 pubmed publisher |
| Awuh J, Haug M, Mildenberger J, Marstad A, Do C, Louet C, et al. Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages. Proc Natl Acad Sci U S A. 2015;112:E4272-80 pubmed publisher |
| Kobayashi S, Koujin T, Kojidani T, Osakada H, Mori C, Hiraoka Y, et al. BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy. Proc Natl Acad Sci U S A. 2015;112:7027-32 pubmed publisher |
| Zlatanou A, Sabbioneda S, Miller E, Greenwalt A, Aggathanggelou A, Maurice M, et al. USP7 is essential for maintaining Rad18 stability and DNA damage tolerance. Oncogene. 2016;35:965-76 pubmed publisher |
| Wright F, Lu J, Sliter D, Dupré N, Rouleau G, Wojcikiewicz R. A Point Mutation in the Ubiquitin Ligase RNF170 That Causes Autosomal Dominant Sensory Ataxia Destabilizes the Protein and Impairs Inositol 1,4,5-Trisphosphate Receptor-mediated Ca2+ Signaling. J Biol Chem. 2015;290:13948-57 pubmed publisher |
| Raval K, Tao R, White B, de Lange W, Koonce C, Yu J, et al. Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes. J Biol Chem. 2015;290:3121-36 pubmed publisher |
| Veena M, Wilken R, Zheng J, Gholkar A, Venkatesan N, Vira D, et al. p16 Protein and gigaxonin are associated with the ubiquitination of NFκB in cisplatin-induced senescence of cancer cells. J Biol Chem. 2014;289:34921-37 pubmed publisher |
| Watanabe M, Funakoshi T, Unuma K, Aki T, Uemura K. Activation of the ubiquitin-proteasome system against arsenic trioxide cardiotoxicity involves ubiquitin ligase Parkin for mitochondrial homeostasis. Toxicology. 2014;322:43-50 pubmed publisher |
| Funakoshi Hirose I, Aki T, Unuma K, Funakoshi T, Noritake K, Uemura K. Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes. Toxicology. 2013;312:74-82 pubmed publisher |
| Aillet F, Lopitz Otsoa F, Egaña I, Hjerpe R, Fraser P, Hay R, et al. Heterologous SUMO-2/3-ubiquitin chains optimize I?B? degradation and NF-?B activity. PLoS ONE. 2012;7:e51672 pubmed publisher |
| Butler L, Densham R, Jia J, Garvin A, Stone H, Shah V, et al. The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response. EMBO J. 2012;31:3918-34 pubmed publisher |
| Sarkar A, Zohn I. Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme. J Cell Biol. 2012;196:789-800 pubmed publisher |
| Wenger T, Terawaki S, Camosseto V, Abdelrassoul R, Mies A, Catalan N, et al. Autophagy inhibition promotes defective neosynthesized proteins storage in ALIS, and induces redirection toward proteasome processing and MHCI-restricted presentation. Autophagy. 2012;8:350-63 pubmed publisher |
| Myeku N, Figueiredo Pereira M. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 2011;286:22426-40 pubmed publisher |
