product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Polyubiquitinylated conjugates monoclonal antibody (FK1)
catalog :
BML-PW8805-0500
quantity :
500 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FK1
reactivity :
spiny dogfish, human, mouse, rat, fruit fly
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, dot blot
more info or order :
citations: 28
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:500; loading ...; fig 4e
Bae D, Moore K, Mella J, Hayashi S, Hollien J. Degradation of Blos1 mRNA by IRE1 repositions lysosomes and protects cells from stress. J Cell Biol. 2019;218:1118-1127 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:500; loading ...; fig 1d
Mao D, Lin G, Tepe B, Zuo Z, Tan K, Senturk M, et al. VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway. Autophagy. 2019;15:1214-1233 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4a
Matějů D, Franzmann T, Patel A, Kopach A, Boczek E, Maharana S, et al. An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function. EMBO J. 2017;36:1669-1687 pubmed publisher
  • western blot; mouse; loading ...; fig 8b
Upadhyay M, Agarwal S, Bhadauriya P, Ganesh S. Loss of laforin or malin results in increased Drp1 level and concomitant mitochondrial fragmentation in Lafora disease mouse models. Neurobiol Dis. 2017;100:39-51 pubmed publisher
  • western blot; human; 1:1000; fig 3
Ma B, Cheng H, Gao R, Mu C, Chen L, Wu S, et al. Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-β signalling pathways. Nat Commun. 2016;7:11123 pubmed publisher
  • western blot; human; fig 4
Jean Charles P, Zhang L, Wu J, Han S, Brian L, Freedman N, et al. Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of β-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor κB (NFκB) Activation. J Biol Chem. 2016;291:7450-64 pubmed publisher
  • western blot; rat; 1:1000; fig 2
  • western blot; mouse; 1:1000; fig 1
Gilda J, Ghosh R, Cheah J, West T, Bodine S, Gomes A. Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS ONE. 2015;10:e0135392 pubmed publisher
  • dot blot; spiny dogfish; 1:2500; loading ...; fig 5c, 6c
MacLellan R, Tunnah L, Barnett D, Wright P, Maccormack T, Currie S. Chaperone roles for TMAO and HSP70 during hyposmotic stress in the spiny dogfish shark (Squalus acanthias). J Comp Physiol B. 2015;185:729-40 pubmed publisher
  • western blot; human; 1:1000; fig 3
Ma B, Chen Y, Chen L, Cheng H, Mu C, Li J, et al. Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase. Nat Cell Biol. 2015;17:95-103 pubmed publisher
  • western blot; human; 1:500
Hans F, Fiesel F, Strong J, J ckel S, Rasse T, Geisler S, et al. UBE2E ubiquitin-conjugating enzymes and ubiquitin isopeptidase Y regulate TDP-43 protein ubiquitination. J Biol Chem. 2014;289:19164-79 pubmed publisher
  • western blot; human
Koschny R, Ganten T, Sykora J, Haas T, Sprick M, Kolb A, et al. TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window. Hepatology. 2007;45:649-58 pubmed
Jia R, Bonifacino J. Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3. elife. 2019;8: pubmed publisher
Tunnah L, MacKellar S, Barnett D, MacCormack T, Stehfest K, Morash A, et al. Physiological responses to hypersalinity correspond to nursery ground usage in two inshore shark species (Mustelus antarcticus and Galeorhinus galeus). J Exp Biol. 2016;219:2028-38 pubmed publisher
Tanaka M, Nakanishi R, Murakami S, Fujita N, Kondo H, Ishihara A, et al. Effectiveness of daily eccentric contractions induced via kilohertz frequency transcutaneous electrical stimulation on muscle atrophy. Acta Histochem. 2016;118:56-62 pubmed publisher
Pecci A, Necchi V, Barozzi S, Vitali A, Boveri E, Elena C, et al. Particulate cytoplasmic structures with high concentration of ubiquitin-proteasome accumulate in myeloid neoplasms. J Hematol Oncol. 2015;8:71 pubmed publisher
Luo X, Wasilko D, Liu Y, Sun J, Wu X, Luo Z, et al. Structure of the Legionella Virulence Factor, SidC Reveals a Unique PI(4)P-Specific Binding Domain Essential for Its Targeting to the Bacterial Phagosome. PLoS Pathog. 2015;11:e1004965 pubmed publisher
Tian X, Gala U, Zhang Y, Shang W, Nagarkar Jaiswal S, di Ronza A, et al. A voltage-gated calcium channel regulates lysosomal fusion with endosomes and autophagosomes and is required for neuronal homeostasis. PLoS Biol. 2015;13:e1002103 pubmed publisher
Tomasovic A, Kurrle N, Sürün D, Heidler J, Husnjak K, Poser I, et al. Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions. J Biol Chem. 2015;290:9738-52 pubmed publisher
Kommaddi R, Jean Charles P, Shenoy S. Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of β2 adrenergic receptors to autophagosomes during physiological stress. J Biol Chem. 2015;290:8888-903 pubmed publisher
Su H, Chen M, Sands J, Chen G. Activation of the cAMP/PKA pathway induces UT-A1 urea transporter monoubiquitination and targets it for lysosomal degradation. Am J Physiol Renal Physiol. 2013;305:F1775-82 pubmed publisher
Tikoo S, Madhavan V, Hussain M, Miller E, Arora P, Zlatanou A, et al. Ubiquitin-dependent recruitment of the Bloom syndrome helicase upon replication stress is required to suppress homologous recombination. EMBO J. 2013;32:1778-92 pubmed publisher
Cronin Furman E, Borland M, Bergquist K, Bennett J, Trimmer P. Mitochondrial quality, dynamics and functional capacity in Parkinson's disease cybrid cell lines selected for Lewy body expression. Mol Neurodegener. 2013;8:6 pubmed publisher
Sarkar A, Zohn I. Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme. J Cell Biol. 2012;196:789-800 pubmed publisher
Haegens A, Schols A, Gorissen S, van Essen A, Snepvangers F, Gray D, et al. NF-?B activation and polyubiquitin conjugation are required for pulmonary inflammation-induced diaphragm atrophy. Am J Physiol Lung Cell Mol Physiol. 2012;302:L103-10 pubmed publisher
Ma M, Heath C, Hair A, West A. Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity. PLoS Genet. 2011;7:e1002175 pubmed publisher
Mostowy S, Sancho Shimizu V, Hamon M, Simeone R, Brosch R, Johansen T, et al. p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different autophagy pathways. J Biol Chem. 2011;286:26987-95 pubmed publisher
Laederich M, Degnin C, Lunstrum G, Holden P, Horton W. Fibroblast growth factor receptor 3 (FGFR3) is a strong heat shock protein 90 (Hsp90) client: implications for therapeutic manipulation. J Biol Chem. 2011;286:19597-604 pubmed publisher
Lukas C, Savic V, Bekker Jensen S, Doil C, Neumann B, Pedersen R, et al. 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress. Nat Cell Biol. 2011;13:243-53 pubmed publisher
product information
Product ID :
BML-PW8805
Catalog Number :
BML-PW8805-0500
Product Name :
Polyubiquitinylated conjugates monoclonal antibody (FK1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
500 µg
list of Pubmed id :
26627335, 26081257, 26067986, 25811491, 25716320, 25666616, 24133116, 23708797, 23351342, 22431752
Clone :
FK1
Isotype :
IgM
Product Description :
The hybridoma secreting MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Polyubiquitinylated Conjugates (FK1) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise only polyubiquitinylated proteins and not monoubiquitinylated proteins or free ubiquitin.
SPECIES REACTIVITY :
Species independent
Immunogen :
Poly-ubiquitinylated lysozyme.
Specificity :
Recognizes polyubiquitinylated conjugates.
Buffer :
Liquid. In PBS containing 0.1% sodium azide.
Application Summary :
ELISA, IHC, WB
Storage :
+4°C
more info or order :
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.