Proteasome 19S Rpt5/S6a subunit monoclonal antibody (TBP1-19) | Enzo Life Sciences BML-PW8770-0100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 19S Rpt5/S6a subunit monoclonal antibody (TBP1-19)

catalog :

BML-PW8770-0100

quantity :

100 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TBP1-19

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Enzo Life Sciences product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human	Pitcher D, de Mattos Shipley K, Wang Z, Tzortzis K, Goudevenou K, Flynn H, et al. Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE). Biochim Biophys Acta. 2014;1844:2222-8 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50	Milman P, Woulfe J. Novel variant of neuronal intranuclear rodlet immunoreactive for 40 kDa huntingtin associated protein and ubiquitin in the mouse brain. J Comp Neurol. 2013;521:3832-46 pubmed publisher
western blot; rat; 1:2000	Huang Q, Wang H, Perry S, Figueiredo Pereira M. Negative regulation of 26S proteasome stability via calpain-mediated cleavage of Rpn10 subunit upon mitochondrial dysfunction in neurons. J Biol Chem. 2013;288:12161-74 pubmed publisher
	Pitcher D, de Mattos Shipley K, Tzortzis K, Auner H, Karadimitris A, Kleijnen M. Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure. EBioMedicine. 2015;2:642-8 pubmed publisher
	Bollinger L, Powell J, Houmard J, Witczak C, Brault J. Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux. Obesity (Silver Spring). 2015;23:1185-93 pubmed publisher

product information

Product ID :

BML-PW8770

Catalog Number :

BML-PW8770-0100

Product Name :

Proteasome 19S Rpt5/S6a subunit monoclonal antibody (TBP1-19)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µl

list of Pubmed id :

26288836, 26010327

Clone :

TBP1-19

Isotype :

IgG2b

SPECIES REACTIVITY :

Human, Mouse, Rat, Rabbit

Background :

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATPdependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. In addition to the 20S particle, the 26S complex contains over twenty additional proteins, ranging in molecular weight from 25 to 10kDa, located in a distinct complex called the ‘PA700 proteasome activator’ or the ‘19S complex’, and which determines substrate specificity and provides the multiple enzymatic functions necessary for proteolysis and viability. Systematic analysis of the sub-unit components have revealed at least six members to be ATPases belonging to a new family of ATP-binding proteins, together with a further fifteen subunits that lack the capacity to bind ATP, isopeptidases and several other proteins thought to be responsible for the unfolding of a protein substrate prior to insertion into the proteolytic core of the 20S proteasome.

ALTnames :

TBP1, Tat-binding protein 1, 26S protease regulatory subunit 6A, Proteasome 26 S subunit ATPase 3

Immunogen :

Recombinant full-length human Rpt5.

Specificity :

Recognizes the Rpt5/S6a subunit of the 19S regulatory subunit

Buffer :

Liquid. Contains 10mM sodium azide.

Application Summary :

IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage