Proteasome 20S α1, 2, 3, 5, 6 & 7 subunits monoclonal antibody (MCP231) | Enzo Life Sciences BML-PW8195-0100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 20S α1, 2, 3, 5, 6 & 7 subunits monoclonal antibody (MCP231)

catalog :

BML-PW8195-0100

quantity :

100 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MCP231

reactivity :

fission yeast, human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Oliveira A, Gomes Marcondes M. Metformin treatment modulates the tumour-induced wasting effects in muscle protein metabolism minimising the cachexia in tumour-bearing rats. BMC Cancer. 2016;16:418 pubmed publisher
immunocytochemistry; human; loading ...; fig s2	Janjanam J, Rao G. Novel role of cortactin in G protein-coupled receptor agonist-induced nuclear export and degradation of p21Cip1. Sci Rep. 2016;6:28687 pubmed publisher
immunocytochemistry; human; 1:100	Schraivogel D, Schindler S, Danner J, Kremmer E, Pfaff J, Hannus S, et al. Importin-Î² facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels. Nucleic Acids Res. 2015;43:7447-61 pubmed publisher
western blot; mouse; fig 5	Cha J, Burnum Johnson K, Bartos A, Li Y, Baker E, Tilton S, et al. Muscle Segment Homeobox Genes Direct Embryonic Diapause by Limiting Inflammation in the Uterus. J Biol Chem. 2015;290:15337-49 pubmed publisher
western blot; fission yeast; 1:1000	Penney M, Samejima I, Wilkinson C, McInerny C, Mathiassen S, Wallace M, et al. Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor. PLoS ONE. 2012;7:e50796 pubmed publisher
	Kuo C, Goldberg A. Ubiquitinated proteins promote the association of proteasomes with the deubiquitinating enzyme Usp14 and the ubiquitin ligase Ube3c. Proc Natl Acad Sci U S A. 2017;114:E3404-E3413 pubmed publisher
	Lyupina Y, Zatsepina O, Serebryakova M, Erokhov P, Abaturova S, Kravchuk O, et al. Proteomics of the 26S proteasome in Spodoptera frugiperda cells infected with the nucleopolyhedrovirus, AcMNPV. Biochim Biophys Acta. 2016;1864:738-746 pubmed publisher
	Papaevgeniou N, Sakellari M, Jha S, Tavernarakis N, Holmberg C, Gonos E, et al. 18Î±-Glycyrrhetinic Acid Proteasome Activator Decelerates Aging and Alzheimer's Disease Progression in Caenorhabditis elegans and Neuronal Cultures. Antioxid Redox Signal. 2016;25:855-869 pubmed
	Myeku N, CLELLAND C, Emrani S, Kukushkin N, Yu W, Goldberg A, et al. Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling. Nat Med. 2016;22:46-53 pubmed publisher
	Guo X, Wang X, Wang Z, Banerjee S, Yang J, Huang L, et al. Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Nat Cell Biol. 2016;18:202-12 pubmed publisher
	Wang L, Delahunty C, Fritz Wolf K, Rahlfs S, Helena Prieto J, Yates J, et al. Characterization of the 26S proteasome network in Plasmodium falciparum. Sci Rep. 2015;5:17818 pubmed publisher
	Li X, Li Y, Arendt C, Hochstrasser M. Distinct Elements in the Proteasomal Î²5 Subunit Propeptide Required for Autocatalytic Processing and Proteasome Assembly. J Biol Chem. 2016;291:1991-2003 pubmed publisher
	Sokolova V, Li F, Polovin G, Park S. Proteasome Activation is Mediated via a Functional Switch of the Rpt6 C-terminal Tail Following Chaperone-dependent Assembly. Sci Rep. 2015;5:14909 pubmed publisher
	Tomasovic A, Kurrle N, SÃ¼rÃ¼n D, Heidler J, Husnjak K, Poser I, et al. Sestrin 2 protein regulates platelet-derived growth factor receptor Î² (PdgfrÎ²) expression by modulating proteasomal and Nrf2 transcription factor functions. J Biol Chem. 2015;290:9738-52 pubmed publisher
	Andersen K, Jensen C, Kriegenburg F, Lauridsen A, Gordon C, Hartmann Petersen R. Txl1 and Txc1 are co-factors of the 26S proteasome in fission yeast. Antioxid Redox Signal. 2011;14:1601-8 pubmed publisher

product information

Product ID :

BML-PW8195

Catalog Number :

BML-PW8195-0100

Product Name :

Proteasome 20S α1, 2, 3, 5, 6 & 7 subunits monoclonal antibody (MCP231)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µl

list of Pubmed id :

26170235, 25716320

Clone :

MCP231

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Rat, Yeast, Rabbit, Potato

Background :

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. 120S Proteasomes degrade only unfolded proteins in an energy-independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP-dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome. The hybridoma secreting the antibody to subunits HC2, HC3, HC8, HC9, Iota and Zeta was generated by fusion of spenocytes from Balb/c mice which had recieved repeated immunisation with dinitrophenylated proteasomes.

ALTnames :

Proteasome subunit α type-1, -2, -3, -4, -5 & -6, . Macropain subunits C2, C3, C8, C9, ι & ζ

Immunogen :

Dinitrophenylated proteasomes.

Specificity :

Recognizes the α1, 2, 3, 5, 6 & 7 subunits of the 20S proteasome.

Buffer :

Liquid. In PBS containing 0.01% sodium azide.

Application Summary :

IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage