Proteasome 20S β2i subunit polyclonal antibody | Enzo Life Sciences BML-PW8150-0100 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 20S β2i subunit polyclonal antibody

catalog :

BML-PW8150-0100

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, immunohistochemistry, immunocytochemistry

product information

Product ID :

BML-PW8150

Catalog Number :

BML-PW8150-0100

Product Name :

Proteasome 20S β2i subunit polyclonal antibody

Host species :

Rabbit

Clonality :

Polyclonal

Product Type :

Antibody

Size :

100 µl

list of Pubmed id :

20919990, 20035054, 15253927

SPECIES REACTIVITY :

Human, Mouse

Background :

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. Lmp2, Lmp7 and MECL are interferon gamma-inducible catalytic subunits of the 20S proteasome which may replace the constitutive catalytic subunits, delta, X and Z respectively, during proteasome biogenesis. Lmp2 and Lmp7 alter the cleavage site specificity of the 20S proteasome and are required for the efficient generation of T cell epitopes from a number of viral proteins and for optimal MHC class I cell surface expression.

ALTnames :

Proteasome subunit β type-10, Macropain subunit MECl-1

Immunogen :

Synthetic peptide corresponding to aa 218-236 of mouse proteasome 20S (β2i subunit).

Specificity :

Recognizes the β2i subunit of the 20S proteasome.

Buffer :

Liquid. Antiserum containing 10mM sodium azide.

Uses :

Western blot: The serum was characterised by single dimension SDS-PAGE against murine derived proteasomal preparations followed by Western blotting. A protein of ~29kDa was detected using the antibody at a dilution of 1:500-1:1000 using enhanced chemiluminescent detection. When a mouse liver cell derived lysate is probed a number of protein bands are observed in addition to that at ~29kDa.

Application Summary :

IF, IHC, WB

Storage :

-20°C