Proteasome 20S α4 subunit (human) monoclonal antibody (MCP34) | Enzo Life Sciences BML-PW8120-0100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Proteasome 20S α4 subunit (human) monoclonal antibody (MCP34)

catalog :

BML-PW8120-0100

quantity :

100 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MCP34

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; loading ...; fig 3b western blot; human; 1:2000; fig 1	Welk V, Coux O, Kleene V, Abeza C, Trumbach D, Eickelberg O, et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem. 2016;291:13147-59 pubmed publisher
western blot; human; fig 7b	Hochrainer K, Pejanovic N, Olaseun V, Zhang S, Iadecola C, Anrather J. The ubiquitin ligase HERC3 attenuates NF-ÎºB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation. Nucleic Acids Res. 2015;43:9889-904 pubmed publisher
	Myeku N, Figueiredo Pereira M. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 2011;286:22426-40 pubmed publisher

product information

Product ID :

BML-PW8120

Catalog Number :

BML-PW8120-0100

Product Name :

Proteasome 20S α4 subunit (human) monoclonal antibody (MCP34)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µl

list of Pubmed id :

21536669

Clone :

MCP34

Isotype :

IgG1

SPECIES REACTIVITY :

Human

Background :

Two forms of the protein are produced by alternative splicing of the gene - subunits XAPC7-S and XAPC7-L.

ALTnames :

Proteasome subunit α type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7

Immunogen :

Human placenta derived proteasomes.

Specificity :

Recognizes the α4 subunit of the 20S proteasome.

Buffer :

Liquid. In PBS containing 10mM sodium azide.

Uses :

Western blot: Single dimension SDS-PAGE of a human placental proteasome preparation or HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of approximately 28kDa. Upon 2D analysis antibody PW8120 also reacts with two other proteins with the same molecular weight but differing pI. The amount of protein in the spots decreases with their pI. Peptide mapping suggests that the proteins whilst different are closely related. Proteasome preparations containing degradation products often give reaction patterns with several more spots than that shown. Immunoprecipitation: This antibody has been used for immunoprecipitation using standard procedures using immobilised protein A/G for capture. This antibody is also available immobilized on activated agarose (Prod. No. BML-PW9005) and has been used to precipitate proteasomes from a HeLa cell lysate (Prod. No. BML-SW8750).

Application Summary :

IHC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage