product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Ubiquitin monoclonal antibody (P4D1)
catalog :
BML-PW0930-1000
quantity :
1 mg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P4D1
reactivity :
human, mouse, dogs, fruit fly
application :
western blot, immunohistochemistry, immunoprecipitation
more info or order :
citations: 11
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; fig sy5y
López Doménech G, Howden J, Covill Cooke C, Morfill C, Patel J, Bürli R, et al. Loss of neuronal Miro1 disrupts mitophagy and induces hyperactivation of the integrated stress response. EMBO J. 2021;40:e100715 pubmed publisher
  • western blot; human; 1:1000; fig 2f
Ranjan K, Pathak C. FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis. Sci Rep. 2016;6:22787 pubmed publisher
  • western blot; human; fig 3
Kuo H, Hsu H, Chen Y, Chang Y, Liu F, Wu C. Galectin-3 modulates the EGFR signalling-mediated regulation of Sox2 expression via c-Myc in lung cancer. Glycobiology. 2016;26:155-65 pubmed publisher
  • western blot; fruit fly ; 1:2000
Koppen T, Weckmann A, Muller S, Staubach S, Bloch W, Dohmen R, et al. Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics. 2011;11:4397-410 pubmed publisher
Surana P, Das R. Observing a late folding intermediate of Ubiquitin at atomic resolution by NMR. Protein Sci. 2016;25:1438-50 pubmed publisher
Olsen C, Markussen T, Thiede B, Rimstad E. Infectious Salmon Anaemia Virus (ISAV) RNA Binding Protein Encoded by Segment 8 ORF2 and Its Interaction with ISAV and Intracellular Proteins. Viruses. 2016;8: pubmed publisher
Ranjan K, Pathak C. Expression of cFLIPL Determines the Basal Interaction of Bcl-2 With Beclin-1 and Regulates p53 Dependent Ubiquitination of Beclin-1 During Autophagic Stress. J Cell Biochem. 2016;117:1757-68 pubmed publisher
Kim E, Wang B, Sastry N, Masliah E, Nelson P, Cai H, et al. NEDD4-mediated HSF1 degradation underlies α-synucleinopathy. Hum Mol Genet. 2016;25:211-22 pubmed publisher
Sokolova V, Li F, Polovin G, Park S. Proteasome Activation is Mediated via a Functional Switch of the Rpt6 C-terminal Tail Following Chaperone-dependent Assembly. Sci Rep. 2015;5:14909 pubmed publisher
Teng Y, Rezvani K, De Biasi M. UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP. Biochem Pharmacol. 2015;97:518-530 pubmed publisher
Ranaweera R, Yang X. Auto-ubiquitination of Mdm2 enhances its substrate ubiquitin ligase activity. J Biol Chem. 2013;288:18939-46 pubmed publisher
product information
Product ID :
BML-PW0930
Catalog Number :
BML-PW0930-1000
Product Name :
Ubiquitin monoclonal antibody (P4D1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
1 mg
list of Pubmed id :
26503960, 26265139
Clone :
P4D1
Isotype :
IgG1
SPECIES REACTIVITY :
Species independent
Background :
Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
Immunogen :
Denatured bovine ubiquitin.
Specificity :
Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin.
Buffer :
Liquid. In PBS containing 0.01% sodium azide.
Uses :
Clone P4D1 has been reported to be of utility in immunoprecipitation, immunofluorescence and immunohistochemistry (see Brady et al. (2005); Wang et al. (2008); Ito et al. (2003) respectively).
Application Summary :
IHC, IP, WB
Storage :
-20°C
more info or order :
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.