Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4) | Enzo Life Sciences BML-PW0600-0025 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4)

catalog :

BML-PW0600-0025

quantity :

25 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HWA4C4

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Enzo Life Sciences product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 7f	Su X, Wu C, Ye X, Zeng M, Zhang Z, Che Y, et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis. 2018;9:302 pubmed publisher
western blot; mouse; 1:500; loading ...; fig 1a	Cederquist C, Lentucci C, Martinez Calejman C, Hayashi V, Orofino J, GUERTIN D, et al. Systemic insulin sensitivity is regulated by GPS2 inhibition of AKT ubiquitination and activation in adipose tissue. Mol Metab. 2017;6:125-137 pubmed publisher
western blot; mouse; fig 5	Su X, Yan H, Huang Y, Yun H, Zeng B, Wang E, et al. Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus. Sci Rep. 2015;5:18588 pubmed publisher
western blot; mouse; 1:750; fig 1c	Zhang H, Hu H, Greeley N, Jin J, Matthews A, Ohashi E, et al. STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13. Nat Commun. 2014;5:5798 pubmed publisher
immunocytochemistry; human	Xue J, Lv D, Jiao S, Zhao W, Li X, Sun H, et al. pVHL mediates K63-linked ubiquitination of nCLU. PLoS ONE. 2012;7:e35848 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Paine S, Bedford L, Thorpe J, Mayer R, Cavey J, Bajaj N, et al. Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration. Neurosci Lett. 2009;460:205-8 pubmed publisher
	Aripaka K, Gudey S, Zang G, Schmidt A, Åhrling S, Österman L, et al. TRAF6 function as a novel co-regulator of Wnt3a target genes in prostate cancer. EBioMedicine. 2019;: pubmed publisher
	Kells Andrews R, Margraf R, Fisher C, Falk M. Connexin-43 K63-polyubiquitylation on lysines 264 and 303 regulates gap junction internalization. J Cell Sci. 2018;131: pubmed publisher
	Gainullin M, Zhukov I, Zhou X, Mo Y, Astakhova L, Ernberg I, et al. Degradation of cofilin is regulated by Cbl, AIP4 and Syk resulting in increased migration of LMP2A positive nasopharyngeal carcinoma cells. Sci Rep. 2017;7:9012 pubmed publisher
	Zhao Y, Ma C, Wu L, Iwai K, Ashwell J, Oltz E, et al. CYLD and the NEMO Zinc Finger Regulate Tumor Necrosis Factor Signaling and Early Embryogenesis. J Biol Chem. 2015;290:22076-84 pubmed publisher
	Lim G, Chua D, Basil A, Chan H, Chai C, Arumugam T, et al. Cytosolic PTEN-induced Putative Kinase 1 Is Stabilized by the NF-ÎºB Pathway and Promotes Non-selective Mitophagy. J Biol Chem. 2015;290:16882-93 pubmed publisher
	Paine S, Anderson G, Bedford K, Lawler K, Mayer R, Lowe J, et al. Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of ?-synuclein. PLoS ONE. 2013;8:e54711 pubmed publisher
	Zhou F, Zhang X, van Dam H, Ten Dijke P, Huang H, Zhang L. Ubiquitin-specific protease 4 mitigates Toll-like/interleukin-1 receptor signaling and regulates innate immune activation. J Biol Chem. 2012;287:11002-10 pubmed publisher
	Mirzaei H, Rogers R, Grimes B, Eng J, Aderem A, Aebersold R. Characterizing the connectivity of poly-ubiquitin chains by selected reaction monitoring mass spectrometry. Mol Biosyst. 2010;6:2004-14 pubmed publisher

product information

Product ID :

BML-PW0600

Catalog Number :

BML-PW0600-0025

Product Name :

Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

25 µl

list of Pubmed id :

26224629, 25987559, 23382946

Clone :

HWA4C4

Isotype :

IgG2a

Product Description :

Modification of proteins by addition of K63-linked polyubiquitin chains is implicated in a variety of cellular events, including DNA repair, signal transduction and receptor endocytosis. A monoclonal antibody (mAb) has been generated that specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitinspecific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells. The hybridoma secreting the antibody [clone HWA4C4] was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif. Immunoglobulin was purified from tissue culture supernatant by ion exchange chromatography.

SPECIES REACTIVITY :

Species independent

Immunogen :

Synthetic peptide containing the ubiquitin K63-isopeptide motif.

Specificity :

Recognizes K63-linked polyubiquitin in wide range of species.

Buffer :

Liquid. In PBS containing 0.09% sodium azide.

Application Summary :

ELISA, ICC, IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage