This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
protein
product name :
Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (fluorogenic substrate)
catalog :
BML-P276-0001
quantity :
1 mg
citations: 2
Reference
Genna A, Lapetina S, Lukic N, Twafra S, Meirson T, Sharma V, et al. Pyk2 and FAK differentially regulate invadopodia formation and function in breast cancer cells. J Cell Biol. 2018;217:375-395 pubmed publisher
Biela A, Watkinson M, Meier U, Baker D, Giovannoni G, Becer C, et al. Disposable MMP-9 sensor based on the degradation of peptide cross-linked hydrogel films using electrochemical impedance spectroscopy. Biosens Bioelectron. 2015;68:660-667 pubmed publisher
product information
Product ID :
BML-P276
Catalog Number :
BML-P276-0001
Product Name :
Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (fluorogenic substrate)
Product Type :
Peptide
Size :
1 mg
list of Pubmed id :
25660510
Product Description :
Sensitive fluorogenic substrate for most matrix metalloproteinase (MMP) enzymes, as follows [kcat/Km (M-1s-1) and Km (µM), in parentheses]: MMP-1 (9.2 x 104, 27); MMP-2 (6.9x105, >30); MMP-3 (2.0x104); MMP-7 (2.9x105); MMP-8 (4.2x105); MMP-9 (6.2x105, >30); MMP-12 (1.3x105, 130); MMP-13 (1.1x106, 5.2); MMP-14 (1.3x106, 7.9); mouse, rat, and monkey MMP-14; MMP-16; MMP-20; ADAM10 (1.1x103); ADAM17/TACE (7.8x105, 26), and BACE2 (internal data using Prod. No. BML-SE550). It is cleaved very poorly by neutrophil elastase, and not at all by trypsin. Can be used to measure cell-associated activity, or activity in cell-conditioned medium, or in biological fluids such as synovial fluid. Mca fluorescence is quenched by the Dpa group until cleavage separates them (MMPs cleave between Gly-Leu). Ex.: 328nm, Em.: 400nm, but it is possible to use Ex./Em. 320-340/393-420nm. This highly-quenched (98.4%) substrate is useful for inhibitor screening and kinetic analysis.
ALTnames :
FS-6
Purity :
≥98% (HPLC)
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.