FLUOR DE LYS®-HDAC8 deacetylase substrate | Enzo Life Sciences BML-KI178-0005 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

protein

product name :

FLUOR DE LYS®-HDAC8 deacetylase substrate

catalog :

BML-KI178-0005

quantity :

0.5 µmol

citations: 4

Reference
Decroos C, Bowman C, Moser J, Christianson K, Deardorff M, Christianson D. Compromised structure and function of HDAC8 mutants identified in Cornelia de Lange Syndrome spectrum disorders. ACS Chem Biol. 2014;9:2157-64 pubmed publisher
Stolfa D, Marek M, Lancelot J, Hauser A, Walter A, Leproult E, et al. Molecular basis for the antiparasitic activity of a mercaptoacetamide derivative that inhibits histone deacetylase 8 (HDAC8) from the human pathogen schistosoma mansoni. J Mol Biol. 2014;426:3442-53 pubmed publisher
Marek M, Kannan S, Hauser A, Moraes Mourão M, Caby S, Cura V, et al. Structural basis for the inhibition of histone deacetylase 8 (HDAC8), a key epigenetic player in the blood fluke Schistosoma mansoni. PLoS Pathog. 2013;9:e1003645 pubmed publisher
Brunsteiner M, Petukhov P. Insights from comprehensive multiple receptor docking to HDAC8. J Mol Model. 2012;18:3927-39 pubmed publisher

product information

Product ID :

BML-KI178

Catalog Number :

BML-KI178-0005

Product Name :

FLUOR DE LYS®-HDAC8 deacetylase substrate

Product Type :

Peptide

Size :

0.5 µmol

list of Pubmed id :

25075551, 24657767, 24086136, 22431224

Product Description :

FLUOR DE LYS®-HDAC8 is a fluorogenic, diacetylated peptide substrate for HDAC8 (histone deacetylase-8). Based on residues 379-382 of p53 (Arg-His-Lys(Ac)-Lys(Ac)), a site of regulatory acetylation by the p300 and CBP acetyltransferases (lysines 381, 382), it was the best for HDAC8 from among a panel of substrates patterned on p53, histone H3 and histone H4 acetylation sites. FLUOR DE LYS®-HDAC8 is deacetylated by HDAC8 at a rate of more than 10-fold that of the acetylated lysine substrate, FLUOR DE LYS® (BML-KI104; substrates both at 100 µM). Although named because of HDAC8’s preference for it, it is also an excellent substrate for SIRT1 (BMl-SE239; 5x the rate of BML-KI104 at 25 µM, 0.5 mM NAD+), SIRT2 and HeLa Nuclear Extract (BML-KI140; 3x KI-104 rate, 25 µM). Must be used in conjunction with FLUOR DE LYS® Developer II (BML-KI176). Sufficient for 100-200 assays of human recombinant HDAC8 (BML-SE145; 1 U/well, 50-100 µM substrate).

Purity :

≥95%

Buffer :

Supplied as a 5 mM solution (100 µl) in HDAC Assay Buffer.

Storage :

-80°C