α Fodrin monoclonal antibody (AA6) | Enzo Life Sciences BML-FG6090-0500 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

α Fodrin monoclonal antibody (AA6)

catalog :

BML-FG6090-0500

quantity :

500 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AA6

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

Enzo Life Sciences product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig s5	Jackson T, Du L, Janesko Feldman K, Vagni V, Dezfulian C, Poloyac S, et al. The nuclear splicing factor RNA binding motif 5 promotes caspase activation in human neuronal cells, and increases after traumatic brain injury in mice. J Cereb Blood Flow Metab. 2015;35:655-66 pubmed publisher
western blot; human	Ginet V, Pittet M, Rummel C, Osterheld M, Meuli R, Clarke P, et al. Dying neurons in thalamus of asphyxiated term newborns and rats are autophagic. Ann Neurol. 2014;76:695-711 pubmed publisher
	Sabirzhanov B, Stoica B, Zhao Z, Loane D, Wu J, Dorsey S, et al. miR-711 upregulation induces neuronal cell death after traumatic brain injury. Cell Death Differ. 2016;23:654-68 pubmed publisher
	Cho K, Cho M, Seo J, Peak J, Kong K, Yoon S, et al. Calpain-mediated cleavage of DARPP-32 in Alzheimer's disease. Aging Cell. 2015;14:878-86 pubmed publisher
	Zhang J, Wu F, Gu X, Jin Z, Kong L, Zhang Y, et al. The Blockade of Transmembrane Cl⁻ Flux Mitigates I/R-Induced Heart Injury via the Inhibition of Calpain Activity. Cell Physiol Biochem. 2015;35:2121-34 pubmed publisher

product information

Product ID :

BML-FG6090

Catalog Number :

BML-FG6090-0500

Product Name :

α Fodrin monoclonal antibody (AA6)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

500 µl

list of Pubmed id :

26470728, 26178297, 25896056, 25586139

Clone :

AA6

Isotype :

IgG1

SPECIES REACTIVITY :

Mammalian, Chicken

Background :

Fodrin, also referred to as non-erythroid (αII-; brain) spectrin, is a tetrameric (αγ)2 actin-binding, fibrous protein, widely distributed in vertebrates, which forms part of the sub-membranous cytoskeleton within many cell types including neurons, and is particularly abundant with axons. The α-subunits of fodrins and spectrin are highly conserved phylogenetically, with the exception of human α-fodrin, which shares only 55-59% homology with erythroid-specific α-spectrins. The β-subunits of spectrin (and γ-subunits of fodrins) are species specific. The interleukin-1 converting enzyme (ICE) family of proteases has been implicated as important effectors of the apoptotic pathway, perhaps acting hierarchically in a protease cascade. Neuronal fodrin is known to be cleaved by calpain following ischaemic insult and it has been proposed that calpain and an unidentified protease play a role in the onset of neuronal death following transient forebrain ischaemia. Recently, an ICE-like protease has been implicated in the early cleavage of fodrin, producing a 150kDa fragment, proximal to CPP32 in Fas-induced and C2-ceramide mediated apoptosis. A cleavage product of α-fodrin has been proposed as a candidate autoantigen in primary Sjögren’s syndrome and α-fodrin has been shown to be the source of a so-called ‘inhibitory protein factor’ family, members of which have been shown to inhibit both GABA and ATP-dependent glutamate uptake into purified synaptic vesicles.

ALTnames :

αII-spectrin

Immunogen :

Chicken blood cell membranes following hypotonic lysis and mechanical enucleation.

Buffer :

Liquid. In PBS containing 1.0% BSA and 0.09% sodium azide.

Application Summary :

IHC, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage