This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
ELISA/assay
product name :
MitoCapture™ Mitochondrial apoptosis detection kit
catalog :
ALX-850-232-KI01
quantity :
25 tests
citations: 1
Reference
Kaminker P, Kim S, Taylor R, Zebarjadian Y, Funk W, Morin G, et al. TANK2, a new TRF1-associated poly(ADP-ribose) polymerase, causes rapid induction of cell death upon overexpression. J Biol Chem. 2001;276:35891-9 pubmed
product information
Product ID :
ALX-850-232
Catalog Number :
ALX-850-232-KI01
Product Name :
MitoCapture™ Mitochondrial apoptosis detection kit
Product Type :
Kit
Size :
25 tests
list of Pubmed id :
11454873
Product Description :
Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial membrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy and in apoptotic cells. In healthy cells, MitoCapture™ accumulates and aggregates in the mitochondria, giving them a bright red fluorescence. In apoptotic cells, MitoCapture™ cannot aggregate in the mitochondria due to the altered mitochondrial membrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using the FITC channel for green monomers (Ex(max): 488nm; Em(max): 530nm + 30nm), and the PI channel for red aggregates (Ex(max): 488nm; Em(max): 590nm + 42nm). An assay is completed within 20-30 min.
Application Summary :
FC, Fluorescence microscopy, Fluorescent detection
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.