product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
LRRC32 monoclonal antibody (Plato-1)
catalog :
ALX-804-867-C100
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Plato-1
reactivity :
human, mouse, cat
application :
western blot, ELISA, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments
more info or order :
Enzo Life Sciences product webpage
citations: 13
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 2f
  • flow cytometry; mouse; loading ...; fig 2g
Zhang M, Pan X, Fujiwara K, Jurcak N, Muth S, Zhou J, et al. Pancreatic cancer cells render tumor-associated macrophages metabolically reprogrammed by a GARP and DNA methylation-mediated mechanism. Signal Transduct Target Ther. 2021;6:366 pubmed publisher
  • flow cytometry; cat; loading ...; fig 4b
Zhang L, Reckling S, Dean G. Phenotypic and functional analysis of CD1a+ dendritic cells from cats chronically infected with feline immunodeficiency virus. Comp Immunol Microbiol Infect Dis. 2015;42:53-9 pubmed publisher
  • blocking or activating experiments; human; fig 3
Cuende J, Liénart S, Dedobbeleer O, van der Woning B, De Boeck G, Stockis J, et al. Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo. Sci Transl Med. 2015;7:284ra56 pubmed publisher
  • western blot; mouse; 1:500
Carrillo Galvez A, Cobo M, Cuevas Ocaña S, Gutiérrez Guerrero A, Sánchez Gilabert A, Bongarzone P, et al. Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity. Stem Cells. 2015;33:183-95 pubmed publisher
Dedobbeleer O, Stockis J, van der Woning B, Coulie P, Lucas S. Cutting Edge: Active TGF-?1 Released from GARP/TGF-?1 Complexes on the Surface of Stimulated Human B Lymphocytes Increases Class-Switch Recombination and Production of IgA. J Immunol. 2017;199:391-396 pubmed publisher
Hahn S, Neuhoff A, Landsberg J, Schupp J, Eberts D, Leukel P, et al. A key role of GARP in the immune suppressive tumor microenvironment. Oncotarget. 2016;7:42996-43009 pubmed publisher
Huygens C, Liénart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, et al. Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor β1 (TGF-β1) Production in Human Regulatory T Cells. J Biol Chem. 2015;290:20105-16 pubmed publisher
Miller M, Fogle J, Ross P, Tompkins M. Feline glycoprotein A repetitions predominant anchors transforming growth factor beta on the surface of activated CD4(+)CD25(+) regulatory T cells and mediates AIDS lentivirus-induced T cell immunodeficiency. AIDS Res Hum Retroviruses. 2013;29:641-51 pubmed publisher
Battaglia A, Buzzonetti A, Baranello C, Fanelli M, Fossati M, Catzola V, et al. Interleukin-21 (IL-21) synergizes with IL-2 to enhance T-cell receptor-induced human T-cell proliferation and counteracts IL-2/transforming growth factor-?-induced regulatory T-cell development. Immunology. 2013;139:109-20 pubmed publisher
Daniel V, Sadeghi M, Wang H, Opelz G. CD4(+)CD25(+)Foxp3(+)IFN?(+) Treg are immunosuppressive in vitro and increase with intensity of the alloresponse in pretransplant MLC. Transpl Immunol. 2012;27:114-21 pubmed publisher
Wang R, Kozhaya L, Mercer F, Khaitan A, Fujii H, Unutmaz D. Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A. 2009;106:13439-44 pubmed publisher
Tran D, Andersson J, Wang R, Ramsey H, Unutmaz D, Shevach E. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A. 2009;106:13445-50 pubmed publisher
Tran D, Shevach E. Therapeutic potential of FOXP3(+) regulatory T cells and their interactions with dendritic cells. Hum Immunol. 2009;70:294-9 pubmed publisher
product information
Product ID :
ALX-804-867
Catalog Number :
ALX-804-867-C100
Product Name :
LRRC32 monoclonal antibody (Plato-1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
100 µg
list of Pubmed id :
23373523, 23278180, 22954802, 19666573, 19651619, 19236900
Clone :
Plato-1
Isotype :
IgG2b
SPECIES REACTIVITY :
Human, Mouse
Background :
LRRC32 (leucine rich repeat containing 32; also known as GARP or Garpin; Glycoprotein A repetitions predominant) is a glycoprotein expressed on the cell surface of megakaryocytes, platelets and activated regulatory T (Treg) cells.
ALTnames :
Leucine-rich repeat-containing protein 32, GARP, Garpin, Glycoprotein A repetitions predominant
Immunogen :
Recombinant human LRRC32 (aa 20-627).  Detects a band of ~100kDa (Fc Fusion ALX-522-117)  by Western blot. Native protein is ~72kDa.
Buffer :
Liquid. In PBS containing 50% glycerol and 0.02% sodium azide.
Uses :
Cited applications include flow cytometry.
Application Summary :
ELISA, IP, WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.