product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
IL-33 monoclonal antibody (Nessy-1)
catalog :
ALX-804-840-C100
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Nessy-1
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 1l
Zheng J, Yao L, Zhou Y, Gu X, Wang C, Bao K, et al. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death Dis. 2021;12:871 pubmed publisher
  • western blot; human; loading ...; fig 1a
Stav Noraas T, Edelmann R, Poulsen L, Sundnes O, Phung D, Küchler A, et al. Endothelial IL-33 Expression Is Augmented by Adenoviral Activation of the DNA Damage Machinery. J Immunol. 2017;198:3318-3325 pubmed publisher
  • western blot; mouse
Däbritz J, Judd L, Chalinor H, Menheniott T, Giraud A. Altered gp130 signalling ameliorates experimental colitis via myeloid cell-specific STAT3 activation and myeloid-derived suppressor cells. Sci Rep. 2016;6:20584 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 1
Reine T, Vuong T, Rutkovskiy A, Meen A, Vaage J, Jenssen T, et al. Serglycin in Quiescent and Proliferating Primary Endothelial Cells. PLoS ONE. 2015;10:e0145584 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 2Di
Judd L, Heine R, Menheniott T, Buzzelli J, O Brien Simpson N, Pavlic D, et al. Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice. Am J Physiol Gastrointest Liver Physiol. 2016;310:G13-25 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
Chen H, Sun Y, Lai L, Wu H, Xiao Y, Ming B, et al. Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice. Neuroscience. 2015;308:157-68 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500
  • immunocytochemistry; human; 1:1000
Demyanets S, Kaun C, Pentz R, Krychtiuk K, Rauscher S, Pfaffenberger S, et al. Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature. J Mol Cell Cardiol. 2013;60:16-26 pubmed publisher
  • blocking or activating experiments; mouse
Kaieda S, Wang J, Shnayder R, Fishgal N, Hei H, Lee R, et al. Interleukin-33 primes mast cells for activation by IgG immune complexes. PLoS ONE. 2012;7:e47252 pubmed publisher
  • immunohistochemistry - paraffin section; human; 5 ug/ml
  • western blot; human; 5 ug/ml
Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Küchler A, WEISS M, et al. The alarmin IL-33 is a notch target in quiescent endothelial cells. Am J Pathol. 2012;181:1099-111 pubmed publisher
  • immunohistochemistry - frozen section; human
Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T. The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis. Clin Exp Allergy. 2012;42:218-28 pubmed publisher
  • immunohistochemistry; human
  • western blot; human
Granne I, Southcombe J, Snider J, Tannetta D, Child T, Redman C, et al. ST2 and IL-33 in pregnancy and pre-eclampsia. PLoS ONE. 2011;6:e24463 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000
Demyanets S, Konya V, Kastl S, Kaun C, Rauscher S, Niessner A, et al. Interleukin-33 induces expression of adhesion molecules and inflammatory activation in human endothelial cells and in human atherosclerotic plaques. Arterioscler Thromb Vasc Biol. 2011;31:2080-9 pubmed publisher
Huang H, Tsai S, Wu H, Huang H, Hsieh H, Kuo Y, et al. Chronic exposure to high fat diet triggers myelin disruption and interleukin-33 upregulation in hypothalamus. BMC Neurosci. 2019;20:33 pubmed publisher
Travers J, Rochman M, Miracle C, Habel J, Brusilovsky M, Caldwell J, et al. Chromatin regulates IL-33 release and extracellular cytokine activity. Nat Commun. 2018;9:3244 pubmed publisher
Scott I, Majithiya J, Sandén C, Thornton P, Sanders P, Moore T, et al. Interleukin-33 is activated by allergen- and necrosis-associated proteolytic activities to regulate its alarmin activity during epithelial damage. Sci Rep. 2018;8:3363 pubmed publisher
Furukawa S, Moriyama M, Miyake K, Nakashima H, Tanaka A, Maehara T, et al. Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. Sci Rep. 2017;7:42413 pubmed publisher
Stojkovic S, Kaun C, Basílio J, Rauscher S, Hell L, Krychtiuk K, et al. Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation. Sci Rep. 2016;6:25171 pubmed publisher
Porsbjerg C, Baines K, Gibson P, Bergqvist A, Erjefält J, Sverrild A, et al. IL-33 is related to innate immune activation and sensitization to HDM in mild steroid-free asthma. Clin Exp Allergy. 2016;46:564-74 pubmed publisher
O Donnell C, Mahmoud A, Keane J, Murphy C, White D, Carey S, et al. An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer. Br J Cancer. 2016;114:37-43 pubmed publisher
Calvén J, Akbarshahi H, Menzel M, Ayata C, Idzko M, Bjermer L, et al. Rhinoviral stimuli, epithelial factors and ATP signalling contribute to bronchial smooth muscle production of IL-33. J Transl Med. 2015;13:281 pubmed publisher
Lam M, Hull L, Imrie A, Snidvongs K, Chin D, Pratt E, et al. Interleukin-25 and interleukin-33 as mediators of eosinophilic inflammation in chronic rhinosinusitis. Am J Rhinol Allergy. 2015;29:175-81 pubmed publisher
Millar N, Gilchrist D, Akbar M, Reilly J, Kerr S, Campbell A, et al. MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease. Nat Commun. 2015;6:6774 pubmed publisher
Malcolm J, Awang R, Oliver Bell J, Butcher J, Campbell L, Adrados Planell A, et al. IL-33 Exacerbates Periodontal Disease through Induction of RANKL. J Dent Res. 2015;94:968-75 pubmed publisher
Suttle M, Enoksson M, Zoltowska A, Chatterjee M, Nilsson G, Harvima I. Experimentally induced psoriatic lesions associate with rapid but transient decrease in interleukin-33 immunostaining in epidermis. Acta Derm Venereol. 2015;95:536-41 pubmed publisher
Shao D, Perros F, Caramori G, Meng C, Dormuller P, Chou P, et al. Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension. Biochem Biophys Res Commun. 2014;451:8-14 pubmed publisher
Carlock C, Wu J, Zhou C, Tatum K, Adams H, TAN F, et al. Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis. J Immunol. 2014;193:161-9 pubmed publisher
Seltmann J, Werfel T, Wittmann M. Evidence for a regulatory loop between IFN-? and IL-33 in skin inflammation. Exp Dermatol. 2013;22:102-7 pubmed publisher
Roussel L, Farias R, Rousseau S. IL-33 is expressed in epithelia from patients with cystic fibrosis and potentiates neutrophil recruitment. J Allergy Clin Immunol. 2013;131:913-6 pubmed publisher
Yasuda K, Muto T, Kawagoe T, Matsumoto M, Sasaki Y, Matsushita K, et al. Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice. Proc Natl Acad Sci U S A. 2012;109:3451-6 pubmed publisher
Kakkar R, Hei H, Dobner S, Lee R. Interleukin 33 as a mechanically responsive cytokine secreted by living cells. J Biol Chem. 2012;287:6941-8 pubmed publisher
Akcay A, Nguyen Q, He Z, Turkmen K, Won Lee D, Hernando A, et al. IL-33 exacerbates acute kidney injury. J Am Soc Nephrol. 2011;22:2057-67 pubmed publisher
Ali S, Mohs A, Thomas M, Klare J, Ross R, Schmitz M, et al. The dual function cytokine IL-33 interacts with the transcription factor NF-?B to dampen NF-?B-stimulated gene transcription. J Immunol. 2011;187:1609-16 pubmed publisher
Ohno T, Oboki K, Morita H, Kajiwara N, Arae K, Tanaka S, et al. Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. PLoS ONE. 2011;6:e18404 pubmed publisher
Louten J, Rankin A, Li Y, Murphy E, Beaumont M, Moon C, et al. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation. Int Immunol. 2011;23:307-15 pubmed publisher
product information
Product ID :
ALX-804-840
Catalog Number :
ALX-804-840-C100
Product Name :
IL-33 monoclonal antibody (Nessy-1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
100 µg
list of Pubmed id :
26679377, 26363151, 25857925, 25425229, 25003325, 24860190, 23219168, 22331917, 22215666, 21949094
Clone :
Nessy-1
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat
ALTnames :
Interleukin-33, IL-1F11, NF-HEV
Immunogen :
Recombinant human IL-33 (aa 112-270) (Prod. No. ALX-522-098).
Specificity :
Recognizes pro-IL-33 (precursor) and mature IL-33.
Buffer :
Liquid. In PBS containing 0.02% sodium azide.
Application Summary :
ELISA, IHC (PS), IP, WB
Storage :
-20°C
more info or order :
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.