LAG-3 (human) monoclonal antibody (17B4) (FITC conjugate) | Enzo Life Sciences ALX-804-806F-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

LAG-3 (human) monoclonal antibody (17B4) (FITC conjugate)

catalog :

ALX-804-806F-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

17B4

The same clone is also sold as:

Abnova: MAB16088, MAB16089

reactivity :

human

application :

flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 1	Juno J, Stalker A, Waruk J, Oyugi J, Kimani M, Plummer F, et al. Elevated expression of LAG-3, but not PD-1, is associated with impaired iNKT cytokine production during chronic HIV-1 infection and treatment. Retrovirology. 2015;12:17 pubmed publisher
flow cytometry; human	Juno J, Lajoie J, Stalker A, Oyugi J, Kimani M, Kimani J, et al. Enrichment of LAG-3, but not PD-1, on double negative T cells at the female genital tract. Am J Reprod Immunol. 2014;72:534-40 pubmed publisher
flow cytometry; human; 1:100	Gros A, Robbins P, Yao X, Li Y, Turcotte S, Tran E, et al. PD-1 identifies the patient-specific CD8? tumor-reactive repertoire infiltrating human tumors. J Clin Invest. 2014;124:2246-59 pubmed publisher
	Seifert A, Zeng S, Zhang J, Kim T, Cohen N, Beckman M, et al. PD-1/PD-L1 Blockade Enhances T-cell Activity and Antitumor Efficacy of Imatinib in Gastrointestinal Stromal Tumors. Clin Cancer Res. 2017;23:454-465 pubmed publisher
	Gulati N, SuÃ¡rez FariÃ±as M, Correa Da Rosa J, Krueger J. Psoriasis is characterized by deficient negative immune regulation compared to transient delayed-type hypersensitivity reactions. F1000Res. 2015;4:149 pubmed publisher
	Boer M, Prins C, van Meijgaarden K, van Dissel J, Ottenhoff T, Joosten S. Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults. Clin Vaccine Immunol. 2015;22:778-88 pubmed publisher
	Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
	Hatano R, Ohnuma K, Otsuka H, Komiya E, Taki I, Iwata S, et al. CD26-mediated induction of EGR2 and IL-10 as potential regulatory mechanism for CD26 costimulatory pathway. J Immunol. 2015;194:960-72 pubmed publisher
	Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, et al. Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses. PLoS ONE. 2014;9:e88327 pubmed publisher
	Tan A, Hoang L, Chin D, Rasmussen E, Lopatin U, Hart S, et al. Reduction of HBV replication prolongs the early immunological response to IFN? therapy. J Hepatol. 2014;60:54-61 pubmed publisher
	Sumitomo S, Fujio K, Okamura T, Morita K, Ishigaki K, Suzukawa K, et al. Transcription factor early growth response 3 is associated with the TGF-?1 expression and the regulatory activity of CD4-positive T cells in vivo. J Immunol. 2013;191:2351-9 pubmed publisher
	Radvanyi L, Bernatchez C, Zhang M, Fox P, Miller P, Chacon J, et al. Specific lymphocyte subsets predict response to adoptive cell therapy using expanded autologous tumor-infiltrating lymphocytes in metastatic melanoma patients. Clin Cancer Res. 2012;18:6758-70 pubmed publisher
	Inozume T, Hanada K, Wang Q, Ahmadzadeh M, Wunderlich J, Rosenberg S, et al. Selection of CD8+PD-1+ lymphocytes in fresh human melanomas enriches for tumor-reactive T cells. J Immunother. 2010;33:956-64 pubmed publisher
	Casati C, Camisaschi C, Novellino L, Mazzocchi A, Triebel F, Rivoltini L, et al. Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation. J Immunol. 2008;180:3782-8 pubmed
	Bayry J, Triebel F, Kaveri S, Tough D. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells. J Immunol. 2007;178:4184-93 pubmed
	Biagi E, Rousseau R, Yvon E, Schwartz M, Dotti G, Foster A, et al. Responses to human CD40 ligand/human interleukin-2 autologous cell vaccine in patients with B-cell chronic lymphocytic leukemia. Clin Cancer Res. 2005;11:6916-23 pubmed
	Triebel F, Hacene K, Pichon M. A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors. Cancer Lett. 2006;235:147-53 pubmed

product information

Product ID :

ALX-804-806F

Catalog Number :

ALX-804-806F-C100

Product Name :

LAG-3 (human) monoclonal antibody (17B4) (FITC conjugate)

Host species :

Mouse

Clonality :

Monoclonal

Conjugation :

FITC

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

25810006, 25548232, 25154740, 24667641, 24505475, 20948441, 18322184, 17371975, 16203783, 15946792

Image Legend 4 :

Figure: Expression of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with LAG-3 (human), mAb (17B4) (FITC) (Prod. No. ALX-804-806F).Method: TILs from a dissociated renal cell carcinoma sample, stained with 5µg/ml LAG-3 (human), mAb (17B4) (FITC) (Prod. No. ALX-804-806F) and FITC-coupled anti-CD4 or -CD8, are analyzed by a two-colour FACS analysis. Additional staining with anti-CD3 allowed a gate analysis of total T cells. Values indicate percentages in each quadrant [5].

Clone :

17B4

Isotype :

IgG1

SPECIES REACTIVITY :

Human

Background :

The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.

ALTnames :

Lymphocyte activation gene-3, FDC protein, CD223

Immunogen :

Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).

Specificity :

Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of LAG-3.

Buffer :

Liquid. In PBS containing 50mM Tris-HCl, 1% BSA and 0.02% sodium azide.

Uses :

Excellent for Flow Cytometry.

Application Summary :

Storage :

+4°C

more info or order :

Enzo Life Sciences product webpage