CLIMP-63 monoclonal antibody (G1/296) | Enzo Life Sciences ALX-804-604-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

CLIMP-63 monoclonal antibody (G1/296)

catalog :

ALX-804-604-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

G1/296

reactivity :

African green monkey, human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Enzo Life Sciences product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 3a western blot; human; loading ...; fig 2a immunocytochemistry; African green monkey; loading ...; fig 6a	Gao G, Zhu C, Liu E, Nabi I. Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules. PLoS Biol. 2019;17:e3000355 pubmed publisher
western blot; human; 1:500; loading ...; fig s2a	Simic M, Moehle E, Schinzel R, Lorbeer F, Halloran J, Heydari K, et al. Transient activation of the UPRER is an essential step in the acquisition of pluripotency during reprogramming. Sci Adv. 2019;5:eaaw0025 pubmed publisher
immunocytochemistry; human; fig s5b western blot; human; fig s5d	Khaminets A, Heinrich T, Mari M, Grumati P, Huebner A, Akutsu M, et al. Regulation of endoplasmic reticulum turnover by selective autophagy. Nature. 2015;522:354-8 pubmed publisher
western blot; human	Hashimoto Y, Shirane M, Matsuzaki F, Saita S, Ohnishi T, Nakayama K. Protrudin regulates endoplasmic reticulum morphology and function associated with the pathogenesis of hereditary spastic paraplegia. J Biol Chem. 2014;289:12946-61 pubmed publisher
	Knapp B, Roedig J, Roedig H, Krzysko J, Horn N, Güler B, et al. Affinity Proteomics Identifies Interaction Partners and Defines Novel Insights into the Function of the Adhesion GPCR VLGR1/ADGRV1. Molecules. 2022;27: pubmed publisher
	Chavda B, Ling J, Majernick T, Planey S. Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain. BMC Biochem. 2017;18:13 pubmed publisher
	Nixon Abell J, Obara C, Weigel A, Li D, Legant W, Xu C, et al. Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER. Science. 2016;354: pubmed
	Lange C, Bellecave P, Dao Thi V, Tran H, Penin F, Moradpour D, et al. Determinants for membrane association of the hepatitis C virus NS2 protease domain. J Virol. 2014;88:6519-23 pubmed publisher
	Chang J, Lee S, Blackstone C. Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation. Proc Natl Acad Sci U S A. 2013;110:14954-9 pubmed publisher
	Kohler R, Schmitz D, Cornils H, Hemmings B, Hergovich A. Differential NDR/LATS interactions with the human MOB family reveal a negative role for human MOB2 in the regulation of human NDR kinases. Mol Cell Biol. 2010;30:4507-20 pubmed publisher
	Breuza L, Halbeisen R, Jeno P, Otte S, Barlowe C, Hong W, et al. Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46. J Biol Chem. 2004;279:47242-53 pubmed
	Klopfenstein D, Klumperman J, Lustig A, Kammerer R, Oorschot V, Hauri H. Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment. J Cell Biol. 2001;153:1287-300 pubmed
	Klopfenstein D, Kappeler F, Hauri H. A novel direct interaction of endoplasmic reticulum with microtubules. EMBO J. 1998;17:6168-77 pubmed
	Banham A, Turley H, Pulford K, Gatter K, Mason D. The plasma cell associated antigen detectable by antibody VS38 is the p63 rough endoplasmic reticulum protein. J Clin Pathol. 1997;50:485-9 pubmed
	Schweizer A, Rohrer J, Jeno P, DeMaio A, Buchman T, Hauri H. A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein. J Cell Sci. 1993;104 ( Pt 3):685-94 pubmed
	Schweizer A, Ericsson M, Bachi T, Griffiths G, Hauri H. Characterization of a novel 63 kDa membrane protein. Implications for the organization of the ER-to-Golgi pathway. J Cell Sci. 1993;104 ( Pt 3):671-83 pubmed
	Schweizer A, Rohrer J, Hauri H, Kornfeld S. Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers. J Cell Biol. 1994;126:25-39 pubmed

product information

Product ID :

ALX-804-604

Catalog Number :

ALX-804-604-C100

Product Name :

CLIMP-63 monoclonal antibody (G1/296)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

24648458, 23969831, 20624913, 15308636, 11402071, 9799226, 9378814, 8314870, 8314869, 8027183

Clone :

G1/296

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Monkey

Background :

The cytoskeleton-linking membrane protein of 62kDa (CLIMP-63) is a marker for the reticular subdomain of the rough endoplasmatic reticulum (ER) and is excluded from the outer nuclear membrane. Concentration of CLIMP-63 into patches may enhance microtubule binding on the cytosolic side and contribute to ER morphology by the formation of a protein scaffold in the lumen of the ER, linking the rough ER to microtubules.

ALTnames :

ER protein p63, Cytoskeleton-linking membrane protein of 63kDa

Immunogen :

Human CLIMP-63 (cytoskeleton-linking membrane protein of 63kDa) from Caco-2 cells golgi membrane fraction.

Buffer :

Lyophilized

Application Summary :

ELISA, FC, ICC, IHC (FS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage