Giantin monoclonal antibody (G1/133) | Enzo Life Sciences ALX-804-600-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Giantin monoclonal antibody (G1/133)

catalog :

ALX-804-600-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

G1/133

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Enzo Life Sciences product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; loading ...; fig 6h	Alshafie W, Francis V, Bednarz K, Pan Y, Stroh T, McPherson P. Regulated resurfacing of a somatostatin receptor storage compartment fine-tunes pituitary secretion. J Cell Biol. 2020;219: pubmed publisher
immunohistochemistry; human; loading ...; fig 4d	Horova V, Lyoo H, Różycki B, Chalupska D, Smola M, Humpolickova J, et al. Convergent evolution in the mechanisms of ACBD3 recruitment to picornavirus replication sites. PLoS Pathog. 2019;15:e1007962 pubmed publisher
immunocytochemistry; human; 1:500; fig s2	Pagliuso A, Valente C, Giordano L, Filograna A, Li G, Circolo D, et al. Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase ?. Nat Commun. 2016;7:12148 pubmed publisher
immunocytochemistry; dogs; loading ...; fig 4a	Treyer A, Pujato M, Pechuan X, Müsch A. Iterative sorting of apical and basolateral cargo in Madin-Darby canine kidney cells. Mol Biol Cell. 2016;27:2259-71 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 1	Taverna E, Mora BermÃºdez F, Strzyz P, Florio M, Icha J, Haffner C, et al. Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells. Sci Rep. 2016;6:21206 pubmed publisher
immunocytochemistry; human; loading ...; fig 1b	Tansi F, Kallweit E, Kaether C, Kappe K, Schumann C, Hilger I, et al. Internalization of Near-Infrared Fluorescently Labeled Activatable Cell-Penetrating Peptide and of Proteins into Human Fibrosarcoma Cell Line HT-1080. J Cell Biochem. 2015;116:1222-31 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000	Chen A, Gössling E, Witkowski L, Bhindi A, Bauch C, Roussy G, et al. Regional and subcellular distribution of the receptor-targeting protein PIST in the rat central nervous system. J Comp Neurol. 2012;520:889-913 pubmed publisher
	Pasquier A, Vivot K, Erbs E, Spiegelhalter C, Zhang Z, Aubert V, et al. Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes. Nat Commun. 2019;10:3312 pubmed publisher
	Yaiw K, Mohammad A, Costa H, Taher C, Badrnya S, Assinger A, et al. Human Cytomegalovirus Up-Regulates Endothelin Receptor Type B: Implication for Vasculopathies?. Open Forum Infect Dis. 2015;2:ofv155 pubmed publisher
	Martin L, Fluhrer R, Haass C. Substrate requirements for SPPL2b-dependent regulated intramembrane proteolysis. J Biol Chem. 2009;284:5662-70 pubmed publisher
	Mitrovic S, Ben Tekaya H, Koegler E, Gruenberg J, Hauri H. The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi. Mol Biol Cell. 2008;19:1976-90 pubmed publisher
	Nyfeler B, Michnick S, Hauri H. Capturing protein interactions in the secretory pathway of living cells. Proc Natl Acad Sci U S A. 2005;102:6350-5 pubmed
	Breuza L, Halbeisen R, Jeno P, Otte S, Barlowe C, Hong W, et al. Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46. J Biol Chem. 2004;279:47242-53 pubmed
	Vollenweider F, Kappeler F, Itin C, Hauri H. Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme. J Cell Biol. 1998;142:377-89 pubmed
	Linstedt A, Hauri H. Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa. Mol Biol Cell. 1993;4:679-93 pubmed
	Itin C, Schindler R, Hauri H. Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway. J Cell Biol. 1995;131:57-67 pubmed

product information

Product ID :

ALX-804-600

Catalog Number :

ALX-804-600-C100

Product Name :

Giantin monoclonal antibody (G1/133)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

19114711, 18287528, 15849265, 15308636, 9679138, 7691276, 7559786

Clone :

G1/133

Isotype :

IgG1

SPECIES REACTIVITY :

Monkey, Mammalian, Avian

Background :

Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus.

ALTnames :

Macrogolgin, Golgi complex-associated 372kDa protein, GCP372

Immunogen :

Giantin from Caco-2 cell Golgi membrane fraction.

Buffer :

Lyophilized. Preservative free.

Uses :

Not recommended for Western Blot

Application Summary :

ELISA, FC, ICC, IHC (FS), IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage