RNase L (human) monoclonal antibody (2E9) | Enzo Life Sciences ALX-804-581-C100 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

RNase L (human) monoclonal antibody (2E9)

catalog :

ALX-804-581-C100

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2E9

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 5

Reference
Dong B, Kim S, Hong S, Das Gupta J, Malathi K, Klein E, et al. An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumors. Proc Natl Acad Sci U S A. 2007;104:1655-60 pubmed
Carpten J, Nupponen N, Isaacs S, Sood R, Robbins C, Xu J, et al. Germline mutations in the ribonuclease L gene in families showing linkage with HPC1. Nat Genet. 2002;30:181-4 pubmed
Dong B, Silverman R. Alternative function of a protein kinase homology domain in 2', 5'-oligoadenylate dependent RNase L. Nucleic Acids Res. 1999;27:439-45 pubmed
Wang L, Zhou A, Vasavada S, Dong B, Nie H, Church J, et al. Elevated levels of 2',5'-linked oligoadenylate-dependent ribonuclease L occur as an early event in colorectal tumorigenesis. Clin Cancer Res. 1995;1:1421-8 pubmed
Dong B, Silverman R. 2-5A-dependent RNase molecules dimerize during activation by 2-5A. J Biol Chem. 1995;270:4133-7 pubmed

product information

Product ID :

ALX-804-581

Catalog Number :

ALX-804-581-C100

Product Name :

RNase L (human) monoclonal antibody (2E9)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

17234809, 11799394, 9862963, 9815940, 7876164

Clone :

2E9

Isotype :

IgG1

SPECIES REACTIVITY :

Human

Background :

RNase L is an endoribonuclease regulated by 2-5A synthetase (2-5A). It is a 741 aa cytoplasmic and mitochondrial protein in human. RNase L functions in the antiviral and antiproliferative activities of interferons (IFN) and in multiple apoptotic pathways, standing in the first line of the innate cellular defense mechanism induced by the IFN pathway. In its native state, the N-terminal half of RNase L functions as a repressor of the ribonuclease domain in the C-terminal half. Upon binding with high affinity to 2-5A molecules, the inactive monomeric form of RNase L is induced to dimerize into its catalytically active form. Since mutations in RNase L relate to an increase incidence of prostate cancer, the activation of RNase L directly or indirectly suppresses prostate tumorigenesis and/or metastasis. The 2-5A/RNase L pathway is implicated in mediating apoptosis in response to viral infections and to several types of external stimuli. The fungal ribonuclease, α-sarcin, the RNA modyfing enzyme, ricin A chain, and UV light each lead to ribotoxic stress responses involving damage to the 3-end of the large ribosomal RNA. These treatments activate c-jun N-terminal kinases (JNKs). JNK activation in response to UV irradiation has been linked to apoptosis through Bax (requires caspase 3 activity, appearance of cytosolic cytochrome C indicating involvement of mitochondria).

ALTnames :

Ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent), 2-5A-dependent RNase, Ribonuclease 4

Immunogen :

Recombinant human RNase L.

Buffer :

Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.

Application Summary :

ELISA, ICC, IHC (PS), IP, WB

Storage :

-20°C