product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
globo H monoclonal antibody (MBr1)
catalog :
ALX-804-550-C050
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MBr1
reactivity :
Trachemys dorbigni, human, rat, dogs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, blocking or activating experiments, other
more info or order :
Enzo Life Sciences product webpage
citations: 18
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1a
Breiman A, López Robles M, de Carné Trécesson S, Echasserieau K, Bernardeau K, Drickamer K, et al. Carcinoma-associated fucosylated antigens are markers of the epithelial state and can contribute to cell adhesion through CLEC17A (Prolectin). Oncotarget. 2016;7:14064-82 pubmed publisher
  • ELISA; human; loading ...; fig 2a
Zhang D, Huang P, Zou L, Lowary T, Tan M, Jiang X. Tulane virus recognizes the A type 3 and B histo-blood group antigens. J Virol. 2015;89:1419-27 pubmed publisher
  • blocking or activating experiments; Trachemys dorbigni
Benktander J, Angstrom J, Breimer M, Teneberg S. Redefinition of the carbohydrate binding specificity of Helicobacter pylori BabA adhesin. J Biol Chem. 2012;287:31712-24 pubmed publisher
  • flow cytometry; human
Suila H, Pitkänen V, Hirvonen T, Heiskanen A, Anderson H, Laitinen A, et al. Are globoseries glycosphingolipids SSEA-3 and -4 markers for stem cells derived from human umbilical cord blood?. J Mol Cell Biol. 2011;3:99-107 pubmed publisher
Nakao H, Matsumoto S, Nagai Y, Kojima A, Toyoda H, Hashii N, et al. Characterization of glycoproteins expressing the blood group H type 1 epitope on human induced pluripotent stem (hiPS) cells. Glycoconj J. 2017;34:779-787 pubmed publisher
Li D, Breiman A, Le Pendu J, Uyttendaele M. Binding to histo-blood group antigen-expressing bacteria protects human norovirus from acute heat stress. Front Microbiol. 2015;6:659 pubmed publisher
Matsumoto S, Nakao H, Kawabe K, Nonaka M, Toyoda H, Takishima Y, et al. A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells. J Biol Chem. 2015;290:20071-85 pubmed publisher
Nyström K, Abrantes J, Lopes A, Le Moullac Vaidye B, Marchandeau S, Rocher J, et al. Neofunctionalization of the Sec1 α1,2fucosyltransferase paralogue in leporids contributes to glycan polymorphism and resistance to rabbit hemorrhagic disease virus. PLoS Pathog. 2015;11:e1004759 pubmed publisher
Eller C, Yang G, Ouerfelli O, Raines R. Affinity of monoclonal antibodies for Globo-series glycans. Carbohydr Res. 2014;397:1-6 pubmed publisher
Huang C, Thayer D, Chang A, Best M, Hoffmann J, Head S, et al. Carbohydrate microarray for profiling the antibodies interacting with Globo H tumor antigen. Proc Natl Acad Sci U S A. 2006;103:15-20 pubmed
Adobati E, Zacchetti A, Perico M, Cremonesi F, Rasi G, Vallebona P, et al. Expression profile of saccharide epitope CaMBr1 in normal and neoplastic tissue from dogs, cats, and rats: implication for the development of human-derived cancer vaccines. Histochem J. 1999;31:729-37 pubmed
Cordel S, Goupille C, Hallouin F, Meflah K, Le Pendu J. Role for alpha1,2-fucosyltransferase and histo-blood group antigen H type 2 in resistance of rat colon carcinoma cells to 5-fluorouracil. Int J Cancer. 2000;85:142-8 pubmed
Adobati E, Panza L, Russo G, Colnaghi M, Canevari S. In vitro mimicry of CaMBr1 tumor-associated antigen by synthetic oligosaccharides. Glycobiology. 1997;7:173-8 pubmed
Perrone F, Menard S, Canevari S, Calabrese M, Boracchi P, Bufalino R, et al. Prognostic significance of the CaMBr1 antigen on breast carcinoma: relevance of the type of recognised glycoconjugate. Eur J Cancer. 1993;29A:2113-7 pubmed
Martignone S, Menard S, Bedini A, Paccagnella A, Fasolato S, Veggian R, et al. Study of the expression and function of the tumour-associated antigen CaMBr1 in small cell lung carcinomas. Eur J Cancer. 1993;29A:2020-5 pubmed
Mariani Costantini R, Barbanti P, Colnaghi M, Menard S, Clemente C, Rilke F. Reactivity of a monoclonal antibody with tissues and tumors from the human breast. Immunohistochemical localization of a new antigen and clinicopathologic correlations. Am J Pathol. 1984;115:47-56 pubmed
Bremer E, Levery S, Sonnino S, Ghidoni R, Canevari S, Kannagi R, et al. Characterization of a glycosphingolipid antigen defined by the monoclonal antibody MBr1 expressed in normal and neoplastic epithelial cells of human mammary gland. J Biol Chem. 1984;259:14773-7 pubmed
Mariani Costantini R, Colnaghi M, Leoni F, Menard S, Cerasoli S, Rilke F. Immunohistochemical reactivity of a monoclonal antibody prepared against human breast carcinoma. Virchows Arch A Pathol Anat Histopathol. 1984;402:389-404 pubmed
product information
Product ID :
ALX-804-550
Catalog Number :
ALX-804-550-C050
Product Name :
globo H monoclonal antibody (MBr1)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
50 µg
list of Pubmed id :
25875017, 16373501, 10646838, 10585597, 9134423, 8297650, 7506562, 6711680, 6501317, 6426148
Clone :
MBr1
Isotype :
IgM
Product Description :
The murine monoclonal antibody to globo H (clone MBr1) is raised against the breast cancer cell line MCF7 and recognizes a saccharidic epitope (CaMBr1) overexpressed on a high percentage of human breast, ovary lung and prostate carcinomas. This antigen was originally identified on the immunogen as a globo-series glycosphingolipid with a H-like determinant at its terminus (globo-H), identical to GL6 isolated from human teratocarcinoma. MAb to MBr1 was also found to be reactive with different glycoconjugates (glycoproteins, mucins and glycolipids) in breast and small cell lung carcinoma. Retrospective as well as prospective studies suggest that CaMBr1 expression is associated with tumor aggressiveness and survival outcome in breast and small cell lung cancer and that poor prognosis is more frequently associated with CaMBr1 expression on glycoproteins. The epitope recognized by the MAb to MBr1 consists of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal, available as a synthetic oligosaccharide exploitable for the development of new anti-cancer vaccines. Carbohydrate antigens expressed at the cell surface of human cancer cells hold promise as targets for active immunization with vaccines.CaMBR1 is also expressed on some normal tissues, and its expression restricted tissue distribution makes it a suitable target for oncologic clinical applications. In particular the MAb to MBr1 can be succesfully used for immunocytochemical detection of micrometastatic cells.
SPECIES REACTIVITY :
Human, Rat, Dog, Cat
Immunogen :
Membrane preparation from the human breast carcinoma cell line MCF7.
Specificity :
Recognizes membrane bound glycoconjugates (glycoproteins, mucins and glycolipids) expressing the globo H or its tetrasaccharide terminus. Recognizes an epitope consisting of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal.
Buffer :
Liquid. In 50mM PBS, pH 8.0, containing 150mM sodium chloride. Contains no sodium azide.
Uses :
Not recommended for immunoprecipitation.
Application Summary :
FC, ICC, IHC (FS), IHC (PS), WB
Storage :
+4°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.