BimS/EL/L monoclonal antibody (3C5) | Enzo Life Sciences ALX-804-527-C100 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

BimS/EL/L monoclonal antibody (3C5)

catalog :

ALX-804-527-C100

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3C5

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, other, western blot knockout validation

more info or order :

Enzo Life Sciences product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 2c immunoprecipitation; mouse; fig 2c	Merino D, Giam M, Hughes P, Siggs O, Heger K, O Reilly L, et al. The role of BH3-only protein Bim extends beyond inhibiting Bcl-2-like prosurvival proteins. J Cell Biol. 2009;186:355-62 pubmed publisher
western blot; mouse; loading ...; fig 7e	Hayakawa K, Formica A, Brill Dashoff J, Shinton S, Ichikawa D, Zhou Y, et al. Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. J Exp Med. 2016;213:3007-3024 pubmed
other; mouse; loading ...; fig s4	Schuler F, Baumgartner F, Klepsch V, Chamson M, MÃ¼ller Holzner E, Watson C, et al. The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland. Cell Death Differ. 2016;23:41-51 pubmed publisher
western blot; human; fig s4	Haschka M, Soratroi C, Kirschnek S, Hacker G, Hilbe R, Geley S, et al. The NOXA-MCL1-BIM axis defines lifespan on extended mitotic arrest. Nat Commun. 2015;6:6891 pubmed publisher
western blot; human; 1:1000	Annibaldi A, Heulot M, Martinou J, Widmann C. TAT-RasGAP317-326-mediated tumor cell death sensitization can occur independently of Bax and Bak. Apoptosis. 2014;19:719-33 pubmed publisher
	Happo L, Cragg M, Phipson B, Haga J, Jansen E, Herold M, et al. Maximal killing of lymphoma cells by DNA damage-inducing therapy requires not only the p53 targets Puma and Noxa, but also Bim. Blood. 2010;116:5256-67 pubmed publisher
	O Reilly L, Kruse E, Puthalakath H, Kelly P, Kaufmann T, Huang D, et al. MEK/ERK-mediated phosphorylation of Bim is required to ensure survival of T and B lymphocytes during mitogenic stimulation. J Immunol. 2009;183:261-9 pubmed publisher
	Banerjee A, Grumont R, Gugasyan R, White C, Strasser A, Gerondakis S. NF-kappaB1 and c-Rel cooperate to promote the survival of TLR4-activated B cells by neutralizing Bim via distinct mechanisms. Blood. 2008;112:5063-73 pubmed publisher
	Labi V, Erlacher M, Kiessling S, Manzl C, Frenzel A, O Reilly L, et al. Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development. J Exp Med. 2008;205:641-55 pubmed publisher
	Puthalakath H, O Reilly L, Gunn P, Lee L, Kelly P, Huntington N, et al. ER stress triggers apoptosis by activating BH3-only protein Bim. Cell. 2007;129:1337-49 pubmed
	Kuroda J, Kimura S, Strasser A, Andreeff M, O Reilly L, Ashihara E, et al. Apoptosis-based dual molecular targeting by INNO-406, a second-generation Bcr-Abl inhibitor, and ABT-737, an inhibitor of antiapoptotic Bcl-2 proteins, against Bcr-Abl-positive leukemia. Cell Death Differ. 2007;14:1667-77 pubmed
	Kuroda J, Puthalakath H, Cragg M, Kelly P, Bouillet P, Huang D, et al. Bim and Bad mediate imatinib-induced killing of Bcr/Abl+ leukemic cells, and resistance due to their loss is overcome by a BH3 mimetic. Proc Natl Acad Sci U S A. 2006;103:14907-12 pubmed
	Liston A, Lesage S, Gray D, O Reilly L, Strasser A, Fahrer A, et al. Generalized resistance to thymic deletion in the NOD mouse; a polygenic trait characterized by defective induction of Bim. Immunity. 2004;21:817-30 pubmed

product information

Product ID :

ALX-804-527

Catalog Number :

ALX-804-527-C100

Product Name :

BimS/EL/L monoclonal antibody (3C5)

Host species :

Rat

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µg

list of Pubmed id :

26045049, 20829369, 19651893, 19542438, 18805964, 18299399, 17604722, 17510658, 16997913, 15589170

Clone :

3C5

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Mouse, Rat, Monkey, Dog

ALTnames :

BCL2L11

Immunogen :

Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.

Specificity :

Recognizes an epitope within aa 20-40 of imL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region recently identified.

Buffer :

Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.

Application Summary :

ELISA, FC, ICC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage