Hrs and Hrs-2 monoclonal antibody (A-5) | Enzo Life Sciences ALX-804-382-C050 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Hrs and Hrs-2 monoclonal antibody (A-5)

catalog :

ALX-804-382-C050

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A-5

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 8	Salova A, Belyaeva T, Leontieva E, Zlobina M, Kharchenko M, Kornilova E. Quantum dots implementation as a label for analysis of early stages of EGF receptor endocytosis: a comparative study on cultured cells. Oncotarget. 2016;7:6029-47 pubmed publisher
immunocytochemistry; human	de Carvalho J, de Castro R, da Silva E, Silveira P, da Silva JanuÃ¡rio M, Arruda E, et al. Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection. PLoS ONE. 2014;9:e113691 pubmed publisher
western blot; human	Choy R, Cheng Z, Schekman R. Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid ? (A?) production in the trans-Golgi network. Proc Natl Acad Sci U S A. 2012;109:E2077-82 pubmed publisher
	Boal F, Mansour R, Gayral M, Saland E, Chicanne G, Xuereb J, et al. TOM1 is a PI5P effector involved in the regulation of endosomal maturation. J Cell Sci. 2015;128:815-27 pubmed publisher
	Rayala S, Hollander P, Balasenthil S, Molli P, Bean A, Vadlamudi R, et al. Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK. J Biol Chem. 2006;281:4395-403 pubmed
	Yan Q, Sun W, Kujala P, Lotfi Y, Vida T, Bean A. CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling. Mol Biol Cell. 2005;16:2470-82 pubmed
	Shukla A, Hager H, Corydon T, Bean A, Dahl R, Vajda Z, et al. SNAP-25-associated Hrs-2 protein colocalizes with AQP2 in rat kidney collecting duct principal cells. Am J Physiol Renal Physiol. 2001;281:F546-56 pubmed
	Bean A, Davanger S, Chou M, Gerhardt B, Tsujimoto S, Chang Y. Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15. J Biol Chem. 2000;275:15271-8 pubmed
	Jacobsson G, Bean A, Meister B. Isoform-specific exocytotic protein mRNA expression in hypothalamic magnocellular neurons: regulation after osmotic challenge. Neuroendocrinology. 1999;70:392-401 pubmed
	Tsujimoto S, Bean A. Distinct protein domains are responsible for the interaction of Hrs-2 with SNAP-25. The role of Hrs-2 in 7 S complex formation. J Biol Chem. 2000;275:2938-42 pubmed
	Tsujimoto S, Pelto Huikko M, Aitola M, Meister B, Vik Mo E, Davanger S, et al. The cellular and developmental expression of hrs-2 in rat. Eur J Neurosci. 1999;11:3047-63 pubmed

product information

Product ID :

ALX-804-382

Catalog Number :

ALX-804-382-C050

Product Name :

Hrs and Hrs-2 monoclonal antibody (A-5)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µg

list of Pubmed id :

16352611, 15772161, 11502603, 10809762, 10657732, 10644763, 10510169

Clone :

A-5

Isotype :

IgG2a

SPECIES REACTIVITY :

Human, Mouse, Rat, Monkey

Background :

Hrs/Hgs (hepatocyte growth factor-regulated tyrosine kinase substrate) is a tyrosine-phosphorylated member of a protein family characterized by a conserved 3'-phosphoinositide-interacting RING zinc finger domain, the so called FYVE finger. A variant of Hrs, Hrs-2 is an ATPase that has been shown to play a regulatory role in the docking and/or fusion of synaptic vesicles to plasma membranes through a calcium-regulated interaction with SNAP-25. Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and alpha-adaptin, and results in the inhibition of receptor-mediated endocytosis. Immunoelectron microscopy reveals Hrs-2 localization on the limiting membrane of multivesicular bodies, organelles in the endosomal pathway. It is suggested that Hrs-2 acts to provide communication between endo- and exocytic processes.

ALTnames :

Hepatocyte growth factor-regulated tyrosine kinase substrate, HGS

Immunogen :

Recombinant full length rat Hrs-2 (hepatocyte growth factor-regulated tyrosine kinase substrate).

Specificity :

Recognizes Hrs (HGS) and Hrs-2.

Buffer :

Liquid. In 0.1M PBS, pH 7.2, containing 50% glycerol.

Application Summary :

IHC, IP, WB

Storage :

-20°C

more info or order :

Enzo Life Sciences product webpage