Microcystin-LR monoclonal antibody (MC10E7) | Enzo Life Sciences ALX-804-320-C200 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Microcystin-LR monoclonal antibody (MC10E7)

catalog :

ALX-804-320-C200

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MC10E7

application :

western blot, ELISA, immunohistochemistry

citations: 9

Reference
Wu J, Yuan M, Song Y, Sun F, Han X. MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells. Toxins (Basel). 2015;7:5212-23 pubmed publisher
He Y, Reed S, Bhunia A, Gehring A, Nguyen L, Irwin P. Rapid identification and classification of Campylobacter spp. using laser optical scattering technology. Food Microbiol. 2015;47:28-35 pubmed publisher
Wu J, Shao S, Zhou F, Wen S, Chen F, Han X. Reproductive toxicity on female mice induced by microcystin-LR. Environ Toxicol Pharmacol. 2014;37:1-6 pubmed publisher
Yu H, Kim I, Niessner R, Knopp D. Multiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels. Anal Chim Acta. 2012;750:191-8 pubmed publisher
Meng G, Sun Y, Fu W, Guo Z, Xu L. Microcystin-LR induces cytoskeleton system reorganization through hyperphosphorylation of tau and HSP27 via PP2A inhibition and subsequent activation of the p38 MAPK signaling pathway in neuroendocrine (PC12) cells. Toxicology. 2011;290:218-29 pubmed publisher
Djediat C, Malécot M, de Luze A, Bernard C, Puiseux Dao S, Edery M. Localization of microcystin-LR in medaka fish tissues after cyanotoxin gavage. Toxicon. 2010;55:531-5 pubmed publisher
Gurbuz F, Metcalf J, Karahan A, Codd G. Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey. Sci Total Environ. 2009;407:4038-46 pubmed publisher
Lu H, Choudhuri S, Ogura K, Csanaky I, Lei X, Cheng X, et al. Characterization of organic anion transporting polypeptide 1b2-null mice: essential role in hepatic uptake/toxicity of phalloidin and microcystin-LR. Toxicol Sci. 2008;103:35-45 pubmed publisher
Weller M, Zeck A, Eikenberg A, Nagata S, Ueno Y, Niessner R. Development of a direct competitive microcystin immunoassay of broad specificity. Anal Sci. 2001;17:1445-8 pubmed

product information

Product ID :

ALX-804-320

Catalog Number :

ALX-804-320-C200

Product Name :

Microcystin-LR monoclonal antibody (MC10E7)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

200 µg

list of Pubmed id :

24280256, 22000993, 19837107, 19395066, 18296417, 11783797

Clone :

MC10E7

Isotype :

IgG1

Product Description :

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06µg/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 µg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1µg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.

SPECIES REACTIVITY :

Species independent

Background :

Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.

Immunogen :

Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.

Specificity :

Recognizes all 4-Arg microcystins.

Buffer :

Lyophilized

Uses :

Microcystin concentrations can be measured in the range of ~0.006µg/l to 0.3µg/l. The ELISA sensitivity is about 1’000 times higher compared to UV detection.

Application Summary :

ELISA, IHC (PS), WB

Storage :

-20°C