product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
α-Synuclein (human) monoclonal antibody (15G7)
catalog :
ALX-804-258-L001
quantity :
1 ml
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
15G7
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, immunohistochemistry - free floating section, other
more info or order :
Enzo Life Sciences product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...
Reyes J, Ekmark Lewén S, Perdiki M, Klingstedt T, Hoffmann A, Wiechec E, et al. Accumulation of alpha-synuclein within the liver, potential role in the clearance of brain pathology associated with Parkinson's disease. Acta Neuropathol Commun. 2021;9:46 pubmed publisher
  • other; human; loading ...; fig 4a
  • flow cytometry; human; loading ...; fig 4d
  • immunocytochemistry; human; loading ...; fig 5c
  • western blot; human; 1:250; loading ...; fig 4c,4d,5e,6
Menges S, Minakaki G, Schaefer P, Meixner H, Prots I, Schlötzer Schrehardt U, et al. Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress. Sci Rep. 2017;7:42942 pubmed publisher
  • western blot; human; loading ...; fig 1a
Abdullah R, Patil K, Rosen B, Pal R, Prabhudesai S, Lee S, et al. Subcellular Parkinson's Disease-Specific Alpha-Synuclein Species Show Altered Behavior in Neurodegeneration. Mol Neurobiol. 2017;54:7639-7655 pubmed publisher
  • immunocytochemistry; human; fig 5
  • immunohistochemistry; human; 1:100; fig 1
  • western blot; human; fig 1
Sommer A, Fadler T, Dorfmeister E, Hoffmann A, Xiang W, Winner B, et al. Infiltrating T lymphocytes reduce myeloid phagocytosis activity in synucleinopathy model. J Neuroinflammation. 2016;13:174 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 7
Van Rompuy A, Oliveras Salvá M, Van der Perren A, Corti O, Van den Haute C, Baekelandt V. Nigral overexpression of alpha-synuclein in the absence of parkin enhances alpha-synuclein phosphorylation but does not modulate dopaminergic neurodegeneration. Mol Neurodegener. 2015;10:23 pubmed publisher
  • immunocytochemistry; human; 1:100
  • immunohistochemistry - free floating section; mouse; 1:200
Aelvoet S, Ibrahimi A, Macchi F, Gijsbers R, Van den Haute C, Debyser Z, et al. Noninvasive bioluminescence imaging of α-synuclein oligomerization in mouse brain using split firefly luciferase reporters. J Neurosci. 2014;34:16518-32 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 2
  • western blot; human; 1:30; fig 5
Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, et al. Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells. Mol Cell Neurosci. 2014;62:68-78 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200
Krismer F, Wenning G, Li Y, Poewe W, Stefanova N. Intact olfaction in a mouse model of multiple system atrophy. PLoS ONE. 2013;8:e64625 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:20; fig 5
Amschl D, Neddens J, Havas D, Flunkert S, Rabl R, Römer H, et al. Time course and progression of wild type ?-synuclein accumulation in a transgenic mouse model. BMC Neurosci. 2013;14:6 pubmed publisher
Rabl R, Breitschaedel C, Flunkert S, Duller S, Amschl D, Neddens J, et al. Early start of progressive motor deficits in Line 61 ?-synuclein transgenic mice. BMC Neurosci. 2017;18:22 pubmed publisher
Sturm E, Fellner L, Krismer F, Poewe W, Wenning G, Stefanova N. Neuroprotection by Epigenetic Modulation in a Transgenic Model of Multiple System Atrophy. Neurotherapeutics. 2016;13:871-879 pubmed publisher
Ettle B, Kerman B, Valera E, Gillmann C, Schlachetzki J, Reiprich S, et al. ?-Synuclein-induced myelination deficit defines a novel interventional target for multiple system atrophy. Acta Neuropathol. 2016;132:59-75 pubmed publisher
Fellner L, Kuzdas Wood D, Levin J, Ryazanov S, Leonov A, Griesinger C, et al. Anle138b Partly Ameliorates Motor Deficits Despite Failure of Neuroprotection in a Model of Advanced Multiple System Atrophy. Front Neurosci. 2016;10:99 pubmed publisher
Schafferer S, Khurana R, Refolo V, Venezia S, Sturm E, Piatti P, et al. Changes in the miRNA-mRNA Regulatory Network Precede Motor Symptoms in a Mouse Model of Multiple System Atrophy: Clinical Implications. PLoS ONE. 2016;11:e0150705 pubmed publisher
Kuzdas Wood D, Fellner L, Premstaller M, Borm C, Bloem B, Kirik D, et al. Overexpression of α-synuclein in oligodendrocytes does not increase susceptibility to focal striatal excitotoxicity. BMC Neurosci. 2015;16:86 pubmed publisher
Macchi F, Deleersnijder A, Van den Haute C, Munck S, Pottel H, Michiels A, et al. High-content analysis of α-synuclein aggregation and cell death in a cellular model of Parkinson's disease. J Neurosci Methods. 2016;261:117-27 pubmed publisher
Kohl Z, Ben Abdallah N, Vogelgsang J, Tischer L, Deusser J, Amato D, et al. Severely impaired hippocampal neurogenesis associates with an early serotonergic deficit in a BAC α-synuclein transgenic rat model of Parkinson's disease. Neurobiol Dis. 2016;85:206-217 pubmed publisher
Kuzdas Wood D, Irschick R, Theurl M, Malsch P, Mair N, Mantinger C, et al. Involvement of Peripheral Nerves in the Transgenic PLP-α-Syn Model of Multiple System Atrophy: Extending the Phenotype. PLoS ONE. 2015;10:e0136575 pubmed publisher
Deusser J, Schmidt S, Ettle B, Plötz S, Huber S, Müller C, et al. Serotonergic dysfunction in the A53T alpha-synuclein mouse model of Parkinson's disease. J Neurochem. 2015;135:589-97 pubmed publisher
Aldecoa I, Navarro Otano J, Stefanova N, Sprenger F, Seppi K, Poewe W, et al. Alpha-synuclein immunoreactivity patterns in the enteric nervous system. Neurosci Lett. 2015;602:145-9 pubmed publisher
Xiang W, Menges S, Schlachetzki J, Meixner H, Hoffmann A, Schlötzer Schrehardt U, et al. Posttranslational modification and mutation of histidine 50 trigger alpha synuclein aggregation and toxicity. Mol Neurodegener. 2015;10:8 pubmed publisher
Paumier K, Sukoff Rizzo S, Berger Z, Chen Y, Gonzales C, Kaftan E, et al. Behavioral characterization of A53T mice reveals early and late stage deficits related to Parkinson's disease. PLoS ONE. 2013;8:e70274 pubmed publisher
Boudes M, Uvin P, Pinto S, Voets T, Fowler C, Wenning G, et al. Bladder dysfunction in a transgenic mouse model of multiple system atrophy. Mov Disord. 2013;28:347-55 pubmed publisher
Fellner L, Irschick R, Schanda K, Reindl M, Klimaschewski L, Poewe W, et al. Toll-like receptor 4 is required for ?-synuclein dependent activation of microglia and astroglia. Glia. 2013;61:349-60 pubmed publisher
Klucken J, Poehler A, Ebrahimi Fakhari D, Schneider J, Nuber S, Rockenstein E, et al. Alpha-synuclein aggregation involves a bafilomycin A 1-sensitive autophagy pathway. Autophagy. 2012;8:754-66 pubmed publisher
Nemani V, Lu W, Berge V, Nakamura K, Onoa B, Lee M, et al. Increased expression of alpha-synuclein reduces neurotransmitter release by inhibiting synaptic vesicle reclustering after endocytosis. Neuron. 2010;65:66-79 pubmed publisher
Obi T, Nishioka K, Ross O, Terada T, Yamazaki K, Sugiura A, et al. Clinicopathologic study of a SNCA gene duplication patient with Parkinson disease and dementia. Neurology. 2008;70:238-41 pubmed publisher
Mosharov E, Staal R, Bove J, Prou D, Hananiya A, Markov D, et al. Alpha-synuclein overexpression increases cytosolic catecholamine concentration. J Neurosci. 2006;26:9304-11 pubmed
product information
Product ID :
ALX-804-258
Catalog Number :
ALX-804-258-L001
Product Name :
α-Synuclein (human) monoclonal antibody (15G7)
Host species :
Rat
Clonality :
Monoclonal
Product Type :
Antibody
Size :
1 ml
list of Pubmed id :
26620202, 26496712, 23936403, 23691255, 23426727, 23108585, 22647715, 20152114, 18195271, 16957086
Clone :
15G7
Isotype :
IgG2a
SPECIES REACTIVITY :
Human
Background :
Synuclein was originally identified in Torpedo californica as a small neuroprotein that localized to the nuclear envelope of neurons and to presynaptic nerve termini. The human homolog was initially termed NACP, a precursor protein to NAC (Non-Ab Component), because of its prevalence in amyloid plaques in Alzheimer’s patients. Later, NACP was recognized as being a-synuclein (a-SYN), a 14kDa protein, belonging to the synuclein family of phosphoproteins that also includes: b-synuclein, g-synuclein, and synoretin. a-SYN is recognized as a key component in the development and diagnosis of neurodegenerative synucleinopathic diseases (NSDs), such as Alzheimer’s and Parkinson’s disease. In the past five years, several genetic and post-translational modifications to a-SYN have been elucidated that have been linked to its collaboration in the formation of the classical Lewy bodies (LBs) or Lewy neurites that are associated with neurodegeneration. A study that shed more light on the neurogenesis of autorecessive synucleinopathies (ARSs) was the finding that a-SYN accumulation can occur due to mutations in the E3 ubiquitin ligase. ARSs only account for a small percentage of NSD. Thus the illustration that a-SYN has a high susceptibility to tyrosine nitration may be the key component in understanding the formation of LBs. Due to the chemical structure of a-SYN, tyrosine nitration readily leads to the formation of oligomers via covalent O,O’-dityrosine bonds (cross-linking). Nitrosylated, wild-type, a-SYN products thus form SDS-insoluble, heat-stable aggregates in vitro that may account for a-SYN inclusions in all forms of NSDs.
Immunogen :
Synthetic peptide corresponding to aa 116-131 of human α-synuclein.
Buffer :
Liquid. Hybridoma supernatant containing 0.05% sodium azide.
Application Summary :
IHC (FS), IHC (PS), IP, WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.