product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Caspase-8 (human) monoclonal antibody (12F5)
catalog :
ALX-804-242-C100
quantity :
100 µg
price :
381 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12F5
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
Enzo Life Sciences product webpage
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 2j
Zewinger S, Reiser J, Jankowski V, Alansary D, Hahm E, Triem S, et al. Apolipoprotein C3 induces inflammation and organ damage by alternative inflammasome activation. Nat Immunol. 2020;21:30-41 pubmed publisher
  • western blot; human; loading ...; fig 8c
Lee S, North K, Kim E, Jang E, Obeng E, Lu S, et al. Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations. Cancer Cell. 2018;34:225-241.e8 pubmed publisher
  • western blot; human; fig s3d
Gaidt M, Ebert T, Chauhan D, Ramshorn K, Pinci F, Zuber S, et al. The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3. Cell. 2017;171:1110-1124.e18 pubmed publisher
  • western blot; human; loading ...; fig 1c
Weyhenmeyer B, Noonan J, Würstle M, Lincoln F, Johnston G, Rehm M, et al. Predicting the cell death responsiveness and sensitization of glioma cells to TRAIL and temozolomide. Oncotarget. 2016;7:61295-61311 pubmed publisher
  • western blot; human; loading ...; fig 6a
Moriwaki K, Chan F. Regulation of RIPK3- and RHIM-dependent Necroptosis by the Proteasome. J Biol Chem. 2016;291:5948-59 pubmed publisher
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
Schirmer M, Trentin L, Queudeville M, Seyfried F, Demir S, Tausch E, et al. Intrinsic and chemo-sensitizing activity of SMAC-mimetics on high-risk childhood acute lymphoblastic leukemia. Cell Death Dis. 2016;7:e2052 pubmed publisher
  • western blot; human; fig 4
Cousin F, Jouan Lanhouet S, Théret N, Brenner C, Jouan E, Le Moigne Muller G, et al. The probiotic Propionibacterium freudenreichii as a new adjuvant for TRAIL-based therapy in colorectal cancer. Oncotarget. 2016;7:7161-78 pubmed publisher
  • immunocytochemistry; human; fig 8a
Salva K, Wood G. Epigenetically Enhanced Photodynamic Therapy (ePDT) is Superior to Conventional Photodynamic Therapy for Inducing Apoptosis in Cutaneous T-Cell Lymphoma. Photochem Photobiol. 2015;91:1444-51 pubmed publisher
  • western blot; human
Moriwaki K, Shinzaki S, Miyoshi E. GDP-mannose-4,6-dehydratase (GMDS) deficiency renders colon cancer cells resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor- and CD95-mediated apoptosis by inhibiting complex II formation. J Biol Chem. 2011;286:43123-33 pubmed publisher
  • western blot; human; fig 6
Ashley D, Riffkin C, Lovric M, Mikeska T, Dobrovic A, Maxwell J, et al. In vitro sensitivity testing of minimally passaged and uncultured gliomas with TRAIL and/or chemotherapy drugs. Br J Cancer. 2008;99:294-304 pubmed publisher
  • western blot; human
Strauss G, Westhoff M, Fischer Posovszky P, Fulda S, Schanbacher M, Eckhoff S, et al. 4-hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 2008;15:332-43 pubmed
Bartee M, Dunlap K, Bartee E. Myxoma Virus Induces Ligand Independent Extrinsic Apoptosis in Human Myeloma Cells. Clin Lymphoma Myeloma Leuk. 2016;16:203-12 pubmed publisher
de Almagro M, Goncharov T, Newton K, Vucic D. Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination. Cell Death Dis. 2015;6:e1800 pubmed publisher
Kaemmerer E, Kuhn P, Schneider U, Jeon M, Klaus C, Schiffer M, et al. Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes. World J Gastroenterol. 2015;21:4499-508 pubmed publisher
Nagel S, Keuper M, Zagotta I, Enlund E, Ruperez A, Debatin K, et al. Up-regulation of Bcl-2 during adipogenesis mediates apoptosis resistance in human adipocytes. Mol Cell Endocrinol. 2014;382:368-376 pubmed publisher
Riley J, Hutchinson R, McArt D, Crawford N, Holohan C, Paul I, et al. Prognostic and therapeutic relevance of FLIP and procaspase-8 overexpression in non-small cell lung cancer. Cell Death Dis. 2013;4:e951 pubmed publisher
Conus S, Pop C, Snipas S, Salvesen G, Simon H. Cathepsin D primes caspase-8 activation by multiple intra-chain proteolysis. J Biol Chem. 2012;287:21142-51 pubmed publisher
Ewald F, Ueffing N, Brockmann L, Hader C, Telieps T, Schuster M, et al. The role of c-FLIP splice variants in urothelial tumours. Cell Death Dis. 2011;2:e245 pubmed publisher
Hurwitz J, Stasik I, Kerr E, Holohan C, Redmond K, McLaughlin K, et al. Vorinostat/SAHA-induced apoptosis in malignant mesothelioma is FLIP/caspase 8-dependent and HR23B-independent. Eur J Cancer. 2012;48:1096-107 pubmed publisher
Geering B, Gurzeler U, Federzoni E, Kaufmann T, Simon H. A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils. Blood. 2011;117:5953-62 pubmed publisher
Gillissen B, Wendt J, Richter A, Richter A, Müer A, Overkamp T, et al. Endogenous Bak inhibitors Mcl-1 and Bcl-xL: differential impact on TRAIL resistance in Bax-deficient carcinoma. J Cell Biol. 2010;188:851-62 pubmed publisher
Rebsamen M, Meylan E, Curran J, Tschopp J. The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases. Cell Death Differ. 2008;15:1804-11 pubmed publisher
Goval J, Thielen C, Bourguignon C, Greimers R, Dejardin E, Choi Y, et al. The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP. Haematologica. 2008;93:1169-77 pubmed publisher
product information
Product ID :
ALX-804-242
Catalog Number :
ALX-804-242-C100
Product Name :
Caspase-8 (human) monoclonal antibody (12F5)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
100 µg
List Price (USD) :
381 USD
list of Pubmed id :
26111062, 25914458, 24397922, 24309938, 22528489, 22154545, 22027835, 21478427, 20308427, 18756281
Clone :
12F5
Isotype :
IgG2b
SPECIES REACTIVITY :
Human
ALTnames :
FLICE
Immunogen :
Recombinant human caspase-8.
Specificity :
Recognizes procaspase-8 and active caspase-8. Detects procaspase-8 (p55/54), the intermediate cleavage products (p43/41) and the p18 active subunit of caspase-8 by Western blot.
Buffer :
Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA and 0.01% thimerosal.
Application Summary :
IHC (FS), IP, WB
Storage :
+4°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.