This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Ski monoclonal antibody (G8)
catalog :
ALX-804-221-C100
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
G8

The same clone is also sold as:
reactivity :
human, mouse, chicken
application :
western blot, immunocytochemistry, immunoprecipitation
citations: 2
Reference
Nicol R, Zheng G, Sutrave P, Foster D, Stavnezer E. Association of specific DNA binding and transcriptional repression with the transforming and myogenic activities of c-Ski. Cell Growth Differ. 1999;10:243-54 pubmed
Cohen S, Zheng G, Heyman H, Stavnezer E. Heterodimers of the SnoN and Ski oncoproteins form preferentially over homodimers and are more potent transforming agents. Nucleic Acids Res. 1999;27:1006-14 pubmed
product information
Product ID :
ALX-804-221
Catalog Number :
ALX-804-221-C100
Product Name :
Ski monoclonal antibody (G8)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
100 µg
list of Pubmed id :
10319994, 9927733
Clone :
G8
Isotype :
IgG1
SPECIES REACTIVITY :
Human, Mouse, Rat, Xenopus, Chicken
Background :
Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. It has been shown that Ski associates with Smads2, 3 and 4 proteins in response to TGF-β stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF-β-induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF-β family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation.
Immunogen :
Recombinant v-Ski.
Specificity :
Recognizes cellular and hyperphosphorylated Ski.
Buffer :
Liquid. In PBS containing 0.05% sodium azide.
Uses :
Detects bands of ~95kDa (Ski) and bands of up to ~115kDa (hyperphosphorylated Ski) by Western blot.
Application Summary :
ICC, IP, WB
Storage :
-20°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.