product summary
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company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
Poly(ADP-ribose) monoclonal antibody (10H)
catalog :
ALX-804-220-R100
quantity :
100 µl
price :
360 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10H
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry
more info or order :
Enzo Life Sciences product webpage
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig s3j
Zimmermann M, Murina O, Reijns M, Agathanggelou A, Challis R, Tarnauskaitė Ž, et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature. 2018;559:285-289 pubmed publisher
  • western blot; mouse; fig s5e
Margalef P, Kotsantis P, Borel V, Bellelli R, Panier S, Boulton S. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell. 2018;172:439-453.e14 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2
Bot C, Pfeiffer A, Giordano F, Manjeera D, Dantuma N, Ström L. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J Cell Sci. 2017;130:1134-1146 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 3a
Hanzlikova H, Gittens W, Krejcikova K, Zeng Z, Caldecott K. Overlapping roles for PARP1 and PARP2 in the recruitment of endogenous XRCC1 and PNKP into oxidized chromatin. Nucleic Acids Res. 2017;45:2546-2557 pubmed publisher
  • western blot; human; loading ...; fig 3e
Rizzo A, Iachettini S, Salvati E, Zizza P, Maresca C, D Angelo C, et al. SIRT6 interacts with TRF2 and promotes its degradation in response to DNA damage. Nucleic Acids Res. 2017;45:1820-1834 pubmed publisher
  • western blot; mouse
Namba F, Go H, Murphy J, La P, Yang G, Sengupta S, et al. Expression level and subcellular localization of heme oxygenase-1 modulates its cytoprotective properties in response to lung injury: a mouse model. PLoS ONE. 2014;9:e90936 pubmed publisher
  • immunocytochemistry; human; 1:100
Giansanti V, Rodriguez G, Savoldelli M, Gioia R, Forlino A, Mazzini G, et al. Characterization of stress response in human retinal epithelial cells. J Cell Mol Med. 2013;17:103-15 pubmed publisher
Venkatachalam G, Surana U, Clement M. Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress. Nucleic Acids Res. 2017;45:10564-10582 pubmed publisher
Na T, Ka N, Rhee H, Kyeong D, Kim M, Seong J, et al. Interaction of hepatitis B virus X protein with PARP1 results in inhibition of DNA repair in hepatocellular carcinoma. Oncogene. 2016;35:5435-5445 pubmed publisher
Shrestha E, Hussein M, Savas J, Ouimet M, Barrett T, Leone S, et al. Poly(ADP-ribose) Polymerase 1 Represses Liver X Receptor-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. J Biol Chem. 2016;291:11172-84 pubmed publisher
Solarczyk K, Kordon M, Berniak K, Dobrucki J. Two stages of XRCC1 recruitment and two classes of XRCC1 foci formed in response to low level DNA damage induced by visible light, or stress triggered by heat shock. DNA Repair (Amst). 2016;37:12-21 pubmed publisher
Lee H, Gupta M, Kim D, Hwang J, Kwon B, Lee H. Poly(ADP-ribosyl)ation is involved in pro-survival autophagy in porcine blastocysts. Mol Reprod Dev. 2016;83:37-49 pubmed publisher
Ghonim M, Pyakurel K, Ibba S, Wang J, Rodriguez P, Al Khami A, et al. PARP is activated in human asthma and its inhibition by olaparib blocks house dust mite-induced disease in mice. Clin Sci (Lond). 2015;129:951-62 pubmed publisher
Breton C, Aubry D, Ginet V, Puyal J, Heulot M, Widmann C, et al. Combinative effects of β-Lapachone and APO866 on pancreatic cancer cell death through reactive oxygen species production and PARP-1 activation. Biochimie. 2015;116:141-53 pubmed publisher
Martínez Fernández de la Cámara C, Hernández Pinto A, Olivares González L, Cuevas Martín C, Sánchez Aragó M, Hervás D, et al. Adalimumab Reduces Photoreceptor Cell Death in A Mouse Model of Retinal Degeneration. Sci Rep. 2015;5:11764 pubmed publisher
Zhao L, Zabel M, Wang X, Ma W, Shah P, Fariss R, et al. Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Mol Med. 2015;7:1179-97 pubmed publisher
Nakajima H, Kubo T, Ihara H, Hikida T, Danjo T, Nakatsuji M, et al. Nuclear-translocated Glyceraldehyde-3-phosphate Dehydrogenase Promotes Poly(ADP-ribose) Polymerase-1 Activation during Oxidative/Nitrosative Stress in Stroke. J Biol Chem. 2015;290:14493-503 pubmed publisher
Gerace E, Landucci E, Scartabelli T, Moroni F, Chiarugi A, Pellegrini Giampietro D. Interplay between histone acetylation/deacetylation and poly(ADP-ribosyl)ation in the development of ischemic tolerance in vitro. Neuropharmacology. 2015;92:125-34 pubmed publisher
Gu L, Xu H, Wang F, Xu G, Sinha D, Wang J, et al. Erythropoietin exerts a neuroprotective function against glutamate neurotoxicity in experimental diabetic retina. Invest Ophthalmol Vis Sci. 2014;55:8208-22 pubmed publisher
Batnasan E, Wang R, Wen J, Ke Y, Li X, Bohio A, et al. 17-beta estradiol inhibits oxidative stress-induced accumulation of AIF into nucleolus and PARP1-dependent cell death via estrogen receptor alpha. Toxicol Lett. 2015;232:1-9 pubmed publisher
Cincinelli R, Musso L, Merlini L, Giannini G, Vesci L, Milazzo F, et al. 7-Azaindole-1-carboxamides as a new class of PARP-1 inhibitors. Bioorg Med Chem. 2014;22:1089-103 pubmed publisher
Asher G, Reinke H, Altmeyer M, Gutierrez Arcelus M, Hottiger M, Schibler U. Poly(ADP-ribose) polymerase 1 participates in the phase entrainment of circadian clocks to feeding. Cell. 2010;142:943-53 pubmed publisher
Liu X, Palma J, Kinders R, Shi Y, Donawho C, Ellis P, et al. An enzyme-linked immunosorbent poly(ADP-ribose) polymerase biomarker assay for clinical trials of PARP inhibitors. Anal Biochem. 2008;381:240-7 pubmed publisher
product information
Product ID :
ALX-804-220
Catalog Number :
ALX-804-220-R100
Product Name :
Poly(ADP-ribose) monoclonal antibody (10H)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
100 µl
List Price (USD) :
360 USD
list of Pubmed id :
26630398, 26205779, 26188110, 26170250, 25882840, 25623965, 25335981, 25280774, 24398383, 23205553
Image Legend 4 :
Figure 1: Detection of DNA damage.
Clone :
10H
Isotype :
IgG3
Product Description :
The monoclonal antibody 10H is directed against poly(ADP-ribose) (PAR). PAR is synthesized after activation of the nuclear DNA repair enzyme poly(ADP-ribose)polymerase (PARP). PARP is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself.The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP-ribose)glycohydrolase (PARG). After massive DNA damage (e.g. γ-irradiation or oxidative stress) PAR is detectable in the first 10 minutes and disappears later on. In keratinocytes MAb 10H has been shown to detect UVB-induced apoptosis as early as 4 hour after irradiation, thus being superior to DNA laddering and the TUNEL assay.Due to the very large number of endonuclease-mediated DNA breaks in apoptosis, PARP becomes strongly activated during the so-called execution phase. In the case of DNA damage-induced apoptosis, this represents a "second round" of PAR synthesis. PAR synthesized during apoptosis appears to be remarkably stable. PAR immunofluorescence appears at least as early during apoptosis as does the specific cleavage of PARP by caspase-3. As shown by several groups, this PAR immunofluorescence correlates well with other markers of apoptosis. MAb to Poly(ADP-ribose) (10H) can be used in flow cytometry.A quantitative non-isotopic immuno-dot-blot method for the assessment of cellular poly(ADP-ribosyl)ation capacity using MAb to Poly(ADP-ribose) (10H) has been described.
SPECIES REACTIVITY :
Human, Mouse, Rat, Drosophila
ALTnames :
PAR
Immunogen :
Purified poly(ADP-ribose).
Specificity :
Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme.
Buffer :
Liquid. In 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Application Summary :
FC, ICC, IHC (PS), WB
Storage :
-20°C
more info or order :
Enzo Life Sciences product webpage
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.