HIF-1α monoclonal antibody (mgc3) | Enzo Life Sciences ALX-804-216-R100 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

HIF-1α monoclonal antibody (mgc3)

catalog :

ALX-804-216-R100

quantity :

100 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mgc3

The same clone is also sold as:

Abcam: ab16066
Invitrogen: 17-7528-80, 12-7528-82, 17-7528-82, MA1-516

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 3

Reference
Bernhardt W, Campean V, Kany S, Jurgensen J, Weidemann A, Warnecke C, et al. Preconditional activation of hypoxia-inducible factors ameliorates ischemic acute renal failure. J Am Soc Nephrol. 2006;17:1970-8 pubmed
Chilov D, Camenisch G, Kvietikova I, Ziegler U, Gassmann M, Wenger R. Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha. J Cell Sci. 1999;112 ( Pt 8):1203-12 pubmed
Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, et al. General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha. FASEB J. 1999;13:81-8 pubmed

product information

Product ID :

ALX-804-216

Catalog Number :

ALX-804-216-R100

Product Name :

HIF-1α monoclonal antibody (mgc3)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µl

list of Pubmed id :

16762988, 10085255, 9872932

Clone :

mgc3

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Mouse, Primate, Porcine, Bovine

Background :

Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1α (HIF-1α) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1α rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1β.

ALTnames :

Hypoxia-inducible factor 1α, ARNT-interacting protein

Immunogen :

Human HIF-1α (hypoxia-inducible factor 1α) (aa 530-826).

Buffer :

Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.

Uses :

Detects a band of ~116kDa by Western blot after hypoxic induction. Gel Supershift Assay: detects also mouse HIF-1α

Application Summary :

ICC, IP, WB, Gel Supershift

Storage :

-20°C