Apurinic/apyrimidinic endonuclease (human) monoclonal antibody (13B 8E5C2) | Enzo Life Sciences ALX-804-213-R100 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

Apurinic/apyrimidinic endonuclease (human) monoclonal antibody (13B 8E5C2)

catalog :

ALX-804-213-R100

quantity :

100 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B 8E5C2

The same clone is also sold as:

Invitrogen: MA1-440

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation

citations: 2

Reference
Kelley M, Cheng L, Foster R, Tritt R, Jiang J, Broshears J, et al. Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer. Clin Cancer Res. 2001;7:824-30 pubmed
Moore D, Michael H, Tritt R, Parsons S, Kelley M. Alterations in the expression of the DNA repair/redox enzyme APE/ref-1 in epithelial ovarian cancers. Clin Cancer Res. 2000;6:602-9 pubmed

product information

Product ID :

ALX-804-213

Catalog Number :

ALX-804-213-R100

Product Name :

Apurinic/apyrimidinic endonuclease (human) monoclonal antibody (13B 8E5C2)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

100 µl

list of Pubmed id :

11309329, 10690545

Clone :

13B 8E5C2

Isotype :

IgG2a

SPECIES REACTIVITY :

Human

Background :

Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κ B, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5’ of an AP site to produce a hydroxyl group at the 3’ terminus of an unmodified nucleotide upstream of the nick and a 5’-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5’-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3’-5’ DNA exonuclease activity, a 3’-phosphodiesterase activity, a 3’-phosphatase activity, and a RNase H activity. It has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3’ termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.

ALTnames :

HAP1, APEX, APE1, Ref-1

Immunogen :

Recombinant human APE1 (apurinic/apyrimidinic endonuclease).

Buffer :

Liquid. In PBS containing 0.05% sodium azide.

Uses :

Detects a band of ~37kDa by Western blot.

Application Summary :

IHC (PS), IP, WB

Storage :

-20°C