PARP-1 monoclonal antibody (F1-23) | Enzo Life Sciences ALX-804-211-R050 product information

product summary

company name :

Enzo Life Sciences

other brands :

Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

product type :

antibody

product name :

PARP-1 monoclonal antibody (F1-23)

catalog :

ALX-804-211-R050

quantity :

50 µl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F1-23

reactivity :

human, bovine

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Enzo Life Sciences product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 5a	Slyskova J, Sabatella M, Ribeiro Silva C, Stok C, Theil A, Vermeulen W, et al. Base and nucleotide excision repair facilitate resolution of platinum drugs-induced transcription blockage. Nucleic Acids Res. 2018;46:9537-9549 pubmed publisher
immunoprecipitation; human	Rossi M, Carbone M, Mostocotto C, Mancone C, Tripodi M, Maione R, et al. Mitochondrial localization of PARP-1 requires interaction with mitofilin and is involved in the maintenance of mitochondrial DNA integrity. J Biol Chem. 2009;284:31616-24 pubmed publisher
	Zilio N, Williamson C, Eustermann S, Shah R, West S, Neuhaus D, et al. DNA-dependent SUMO modification of PARP-1. DNA Repair (Amst). 2013;12:761-73 pubmed publisher
	Ouararhni K, Hadj Slimane R, Ait Si Ali S, Robin P, Mietton F, Harel Bellan A, et al. The histone variant mH2A1.1 interferes with transcription by down-regulating PARP-1 enzymatic activity. Genes Dev. 2006;20:3324-36 pubmed
	Augustin A, Spenlehauer C, Dumond H, Menissier de Murcia J, Piel M, Schmit A, et al. PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression. J Cell Sci. 2003;116:1551-62 pubmed
	Duriez P, Desnoyers S, Hoflack J, Shah G, Morelle B, Bourassa S, et al. Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase. Biochim Biophys Acta. 1997;1334:65-72 pubmed
	Chinnaiyan A, Tepper C, Seldin M, O Rourke K, Kischkel F, Hellbardt S, et al. FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis. J Biol Chem. 1996;271:4961-5 pubmed
	Kaufmann S, Desnoyers S, Ottaviano Y, Davidson N, Poirier G. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res. 1993;53:3976-85 pubmed
	Lazebnik Y, Kaufmann S, Desnoyers S, Poirier G, Earnshaw W. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature. 1994;371:346-7 pubmed
	Lazebnik Y, Takahashi A, Moir R, Goldman R, Poirier G, Kaufmann S, et al. Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution. Proc Natl Acad Sci U S A. 1995;92:9042-6 pubmed
	Lamarre D, Talbot B, de Murcia G, Laplante C, Leduc Y, Mazen A, et al. Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study. Biochim Biophys Acta. 1988;950:147-60 pubmed

product information

Product ID :

ALX-804-211

Catalog Number :

ALX-804-211-R050

Product Name :

PARP-1 monoclonal antibody (F1-23)

Host species :

Mouse

Clonality :

Monoclonal

Product Type :

Antibody

Size :

50 µl

list of Pubmed id :

23871147, 17158748, 12640039, 9042367, 8617770, 8358726, 8090205, 7568069, 2454668

Clone :

F1-23

Isotype :

IgG1

SPECIES REACTIVITY :

Human, Bovine

ALTnames :

Poly(ADP-ribose) polymerase-1

Immunogen :

Purified bovine PARP-1 (poly(ADP-ribose) polymerase-1).

Specificity :

Recognizes an epitope in the zinc finger II DNA binding domain of PARP-1.

Buffer :

Liquid. Ascites fluid containing 0.02% sodium azide.

Uses :

Detects bands of ~116kDa (intact PARP-1) and ~24kDa (apoptosis-induced cleavage fragment) by Western blot. Functional Application: neutralizes the biological activity of PARP (~80%).

Application Summary :

ELISA, ICC, IP, WB, FUNC, ChIP

Storage :

-80°C

more info or order :

Enzo Life Sciences product webpage