This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Enzo Life Sciences
other brands :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
product type :
antibody
product name :
TNF-R1 (human) monoclonal antibody (H398)
catalog :
ALX-804-200-C100
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
H398

The same clone is also sold as:
reactivity :
human
application :
immunohistochemistry, flow cytometry
citations: 11
Reference
Moh M, Lorenzini P, Gullo C, Schwarz H. Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling. FASEB J. 2013;27:2957-66 pubmed publisher
Gajate C, Mollinedo F. Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy. J Biol Chem. 2005;280:11641-7 pubmed
Lu G, Janjic B, Janjic J, Whiteside T, Storkus W, Vujanovic N. Innate direct anticancer effector function of human immature dendritic cells. II. Role of TNF, lymphotoxin-alpha(1)beta(2), Fas ligand, and TNF-related apoptosis-inducing ligand. J Immunol. 2002;168:1831-9 pubmed
Horie T, Dobashi K, Iizuka K, Yoshii A, Shimizu Y, Nakazawa T, et al. Interferon-gamma rescues TNF-alpha-induced apoptosis mediated by up-regulation of TNFR2 on EoL-1 cells. Exp Hematol. 1999;27:512-9 pubmed
Tang D, Lahti J, Grenet J, Kidd V. Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism. J Biol Chem. 1999;274:7245-52 pubmed
Choi K, Wong F, Harlan J, Chaudhary P, Hood L, Karsan A. Lipopolysaccharide mediates endothelial apoptosis by a FADD-dependent pathway. J Biol Chem. 1998;273:20185-8 pubmed
Geilen C, Bektas M, Wieder T, Kodelja V, Goerdt S, Orfanos C. 1alpha,25-dihydroxyvitamin D3 induces sphingomyelin hydrolysis in HaCaT cells via tumor necrosis factor alpha. J Biol Chem. 1997;272:8997-9001 pubmed
Weigert N, Schaffer K, Schusdziarra V, Classen M, Schepp W. Gastrin secretion from primary cultures of rabbit antral G cells: stimulation by inflammatory cytokines. Gastroenterology. 1996;110:147-54 pubmed
Grell M, Scheurich P, Meager A, Pfizenmaier K. TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res. 1993;12:143-8 pubmed
Pagliacci M, Fumi G, Migliorati G, Grignani F, Riccardi C, Nicoletti I. Cytostatic and cytotoxic effects of tumor necrosis factor alpha on MCF-7 human breast tumor cells are differently inhibited by glucocorticoid hormones. Lymphokine Cytokine Res. 1993;12:439-47 pubmed
Gibbons D, Rowe M, Cope A, Feldmann M, Brennan F. Lymphotoxin acts as an autocrine growth factor for Epstein-Barr virus-transformed B cells and differentiated Burkitt lymphoma cell lines. Eur J Immunol. 1994;24:1879-85 pubmed
product information
Product ID :
ALX-804-200
Catalog Number :
ALX-804-200-C100
Product Name :
TNF-R1 (human) monoclonal antibody (H398)
Host species :
Mouse
Clonality :
Monoclonal
Product Type :
Antibody
Size :
100 µg
list of Pubmed id :
15659383, 11823516, 10089914, 10066786, 9685365, 9083023, 8536851, 8394147, 8123760, 8056047
Clone :
H398
Isotype :
IgG2a
SPECIES REACTIVITY :
Human
ALTnames :
TNF-R 60kDa, CD120a, p55, TNFRSF 1A
Immunogen :
Human TNF-R1.
Specificity :
Recognizes membrane-bound and soluble TNF-R1.
Buffer :
Liquid. In PBS.
Uses :
Not recommended for IHC (PS). Functional Application: blocks TNF-R signalling. 10µg/ml H398 neutralizes TNF induced cytotoxicity on U937 (100%). Furthermore, 10µg/ml H398 neutralizes TNF induced MHC expression on K562, Colo 205 and IL2R on YT cells (always 100%). TNF induced cytotoxicity can be inhibited/neutralized by H398 on MCF-7, HeLa and L929. A working concentration could not be determined, since ascites antibody preparations have been used which exhibit an unspecific cytotoxic effect on the cells. Functional activity via the TNF-R1 is completely inhibited by H398 although on some cells the ligand still binds. Thus, the antibody H398 inhibits signalling via the receptor but not always the binding of TNF.
Application Summary :
FC, IHC (FS), FUNC
Storage :
+4°C
company information
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
info-us@enzolifesciences.
https://www.enzolifesciences.com
1-800-942-0430
headquarters: USA
Enzo Life Sciences, incorporating ALEXIS® Biochemicals and BIOMOL® International, offers a comprehensive panel of innovative life science reagents. Antibodies, enzymes, proteins, small molecule inhibitors, product libraries, and assay kits are available for research in Cancer, Cell Death, Epigenetics, Genomics, Immunology, Neuroscience, Signal Transduction, and Ubiquitin & Proteasomes, Enabling Discovery in Life Science(tm). Visit www.enzolifesciences.com.